推荐产品
生物源
alpaca
品質等級
重組細胞
expressed in E. coli
共軛
unconjugated
抗體表格
affinity purified VHH antibody
無性繁殖
EB02, monoclonal
化驗
>95% (SDS-PAGE)
形狀
lyophilized solid
分子量
13.7 kDa
~15-18 kDa
純化經由
immobilized metal affinity chromatography (IMAC)
物種活性
human
濃度
1 mg/mL
技術
immunofluorescence: 1:200-1:600
immunohistochemistry: 1:200-1:600
顏色
colorless
pH值
( in PBS, pH 7.4)
同型
VHH
Protein ID登錄號
UniProt登錄號
運輸包裝
ambient
儲存溫度
2-8°C
目標翻譯後修改
unmodified
基因資訊
human ... ERBB2(2064)
一般說明
Receptor tyrosine-protein kinase erbB-2, also known as Her2, is a protein that in humans is encoded by the ERBB2 gene. The ErbB family consists of four closely related tyrosine kinase receptors that act as potent mediators of normal cell growth and development. Aberrant expression or function of one or more of these receptors can play a major role in the development and evolution of cancer. Her2 has been implicated in the evolution of both breast and gastric cancers, and is evident in other cancer types such as ovarian and salivary gland tumors. Her2 possesses an active tyrosine kinase domain, but no direct ligand has been identified yet. Her2 is the preferred binding partner to the other members of the ErbB family and is thought to act primarily through the Ras-MAPK, PI3k-PKB/Akt, and PLC-PKC signalling pathways.Alpaca derived anti-HER2 VHH single domain antibody (molecular weight: 13.7 kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. These single-domain antibodies offer superior chemical and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.
特異性
Human Epidermal Growth Factor Receptor 2 (Her2)
免疫原
Recombinant human HER2 (Thr23-Thr652) protein
應用
IHC,IF
包裝
50 µg per vial / 100 µg per vial
外觀
Affinity purified VHH single domain antibody lyophilized from PBS, pH7.8
重構
Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.
儲存和穩定性
Stable for 1 year at 4°C from date of receipt.
其他說明
Storage at 4°C; Do not freeze.
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
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