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Key Documents

SAB4700117

Sigma-Aldrich

Monoclonal Anti-CD20-FITC antibody produced in mouse

clone 2H7, purified immunoglobulin, buffered aqueous solution

别名:

Anti-MS4A1

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About This Item

分類程式碼代碼:
12352203
NACRES:
NA.44

生物源

mouse

共軛

FITC conjugate

抗體表格

purified immunoglobulin

抗體產品種類

primary antibodies

無性繁殖

2H7, monoclonal

形狀

buffered aqueous solution

物種活性

human, nonhuman primates

技術

flow cytometry: suitable

同型

IgG2a

NCBI登錄號

UniProt登錄號

運輸包裝

wet ice

儲存溫度

2-8°C

目標翻譯後修改

unmodified

基因資訊

human ... MS4A1(931)

一般說明

CD20 is a cell surface 33-37 (depending on the degree of phosphorylation) kDa non-glycosylated surface phosphoprotein expressed on mature and most malignant B cells, but not stem cells or plasma cells (low number of the CD20 has been also detected on a subpopulation of T lymphocytes and it can be expressed on follicular dendritic cells). Its expression on B cells is synchronous with the expression of surface IgM. CD20 regulates transmembrane calcium conductance (probably functioning as a component of store-operated calcium channel), cell cycle progression and B-cell proliferation. It is associated with lipid rafts, but the intesity of this association depends on extracellular triggering, employing CD20 conformational change and/or BCR (B cell antigen receptor) aggregation. After the receptor ligation, BCR amd CD20 colocalize and then rapidly dissociate before BCR endocytosis, whereas CD20 remains at the cell surface. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B cells, as it is expressed at high levels on most B-cell malignancies, but does not become internalized or shed from the plasma membrane following mAb treatment.

特異性

The antibody 2H7 reacts with CD20 (Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on B lymphocytes (it is lost on plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes.

免疫原

Human tonsillar B cells

應用

The reagent is designed for Flow Cytometry analysis of human blood cells using 20 μL reagent / 100 μL of whole blood or 1e6 cells in a suspension. The content of a vial (2 mL) is sufficient for 100 tests.

特點和優勢

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

外觀

Solution in phosphate buffered saline containing 15 mM sodium azide and 0.2% high-grade protease free BSA as a stabilizing agent.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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Jessica L Teeling et al.
Journal of immunology (Baltimore, Md. : 1950), 177(1), 362-371 (2006-06-21)
We have previously defined a panel of fully human CD20 mAb. Most of these were unexpectedly efficient in their ability to recruit C1q to the surface of CD20-positive cells and mediate tumor lysis via activation of the classical pathway of

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