推荐产品
生物源
mouse
品質等級
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
F4, monoclonal
形狀
buffered aqueous solution
物種活性
hamster, human
濃度
~1 mg/mL
技術
flow cytometry: suitable
immunoblotting: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
同型
IgG1
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... ABCB1(5243)
一般說明
Anti-P-Glycoprotein (MDR) antibody, mouse monoclonal (mouse IgG1 isotype) is derived from the hybridoma F4 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Multidrug resistance protein 1 (MDR1) is a member of the adenosine triphosphate (ATP)-binding cassette (ABC) transporters superfamily. It is ubiquitously expressed in kidneys, intestines, placenta, liver, adrenal glands blood-brain barrier (BBB), placenta and testis.
特異性
Anti-P-Glycoprotein (MDR) antibody, Mouse monoclonal recognizes an epitope located at the N-terminal region of P-glycoprotein (Pgp), at the third extracellular loop of the molecule, also known as ABCB1 protein. The antibody specifically detects MDR1 P-glycoprotein, but does not recognize MDR3. Anti-P-Glycoprotein (MDR) cross reacts with MDR1 P-glycoprotein from human and hamster origin.
免疫原
a mixture of human and hamster drug-resistant whole cells and crude plasma membranes.
應用
Anti-Glycoprotein (MDR) antibody, mouse monoclonal may be used in:
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunohistochemistry
- cellular enzyme-linked immunosorbent assay (ELISA)
- flow cytometry (FACS)
- cell surface radioimmunoassay (RIA)
生化／生理作用
Multidrug resistance protein 1 (MDR1) functions as an adenosine triphosphate (ATP)-dependent efflux pump. It is also involved in extruding certain xenobiotics and protect cells from toxicants. MDR1 gene is overexpressed in different cancer types including leukemias, lymphomas, myelomas, breast cancer, ovarian cancer, gastrointestinal stromal tumor (GIST), non-small cell lung cancer (NSCLC), fallopian tube, colon, renal, and thyroid cancers. Overexpression of MDR1 gene plays an important role in prompting multidrug resistance in cancer chemotherapy causing high resistance of cancerous cells to a wide variety of substrate anticancer drugs.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
儲存和穩定性
For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.
免責聲明
Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
Gabriella Spengler et al.
Anticancer research, 36(11), 5701-5706 (2016-10-30)
Thioridazine (TZ), an antipsychotic drug, renders multidrug-resistant (MDR) cancer cells susceptible to cytotoxic agents to which they were initially resistant, has anti-prolilferative activity and apoptosis-inducing properties in various tumor cell lines and cancer stem cells. Whereas the anti-proliferative activity takes
Xing-Duo Dong et al.
Frontiers in pharmacology, 13, 843829-843829 (2022-03-15)
The KRAS-G12C inhibitor ARS-1620, is a novel specific covalent inhibitor of KRAS-G12C, possessing a strong targeting inhibitory effect on KRAS-G12C mutant tumors. Overexpression of ATP-binding cassette super-family B member 1 (ABCB1/P-gp) is one of the pivotal factors contributing to multidrug
Multidrug resistance in breast cancer: a meta-analysis of MDR1/gp170 expression and its possible functional significance
Trock B J, et al.
Journal of the National Cancer Institute, 89(13), 917-931 (1997)
Ziyad Binkhathlan et al.
Current cancer drug targets, 13(3), 326-346 (2013-02-02)
One of the major causes of failure in cancer chemotherapy is multidrug resistance (MDR), where cancer cells simultaneously become resistant to different anticancer drugs. Over-expression of membrane efflux pumps like P-glycoprotein (P-gp) that recognizes different chemotherapeutic agents and transports them
The biochemistry of P-glycoprotein-mediated multidrug resistance.
J A Endicott et al.
Annual review of biochemistry, 58, 137-171 (1989-01-01)
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