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生物源
mouse
共軛
unconjugated
抗體表格
purified from hybridoma cell culture
抗體產品種類
primary antibodies
無性繁殖
WASH1-27, monoclonal
形狀
buffered aqueous solution
分子量
antigen ~72 kDa
物種活性
mouse, human
濃度
~1 mg/mL
技術
immunoprecipitation (IP): 5-10 μg using lysates of human HeLa cells
western blot: 2.5-5.0 μg/mL using whole extracts of HEK-293T cells overexpressing mouse WASH1
同型
IgG1
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... WASH1(100287171)
一般說明
Monoclonal Anti-WASH1 (mouse IgG1 isotype) is derived from the hybridoma WASH1-27 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human WASH1. WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), a new member of the Wiskott−Aldrich Syndrome protein (WASP) family. Similar to other WASP family members, it contains a C-terminal for ′WH2, connecting and acidic′ (WCA) domain that binds to actin and the Arp2/3 complex. In addition, WASH1 also contains a short proline-rich region, a unique N-terminal domain termed WASH-homology domain (WAHD1), and a tubulin-binding region. Through its WAHD1 region, it interacts with family with sequence similarity 21 (FAM21), a protein that links WASH1 to endosomes. WASH1 forms part of a multiprotein complex composed of FAM21, KIAA1033 strumpellin and WASH-interacting protein (SWIP) and coiled coil domain containing 53 (CCDC53).
免疫原
a synthetic peptide corresponding to an internal region of human WASH1 (GeneID: 100287171), conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat.
應用
Monoclonal Anti-WASH1 antibody produced in mouse has been used in may be used in various immunochemical techniques including immunoblotting (~72 kDa), immunoprecipitation and immunofluorescence.
生化/生理作用
WASH1 (Wiskott-Aldrich Syndrome Protein and SCAR Homolog), is a nucleation-promoting factor at the surface of endosomes. It recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of endosomes. It associates with tubulin and localizes to early and recycling endosomes, where together with the Arp2/3 complex and actin, it is required for maintaining the shape of the endosomal compartment and the regulation of the retrograde transport.
外觀
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點(°F)
Not applicable
閃點(°C)
Not applicable
Embryonic lethality in mice lacking Trim59 due to impaired gastrulation development
Cell Death & Disease, 9(3), 302-302 (2018)
WASH and the Arp2/3 complex regulate endosome shape and trafficking
Cytoskeleton (Hoboken, N.J.), 67(3), 193-206 (2010)
A FAM21-containing WASH complex regulates retromer-dependent sorting
Developmental Cell, 17(5), 699-711 (2009)
WASH and WAVE actin regulators of the Wiskott-Aldrich syndrome protein (WASP) family are controlled by analogous structurally related complexes
Proceedings of the National Academy of Sciences of the USA, 107(23), 10442-10447 (2010)
The hereditary spastic paraplegia protein strumpellin: characterisation in neurons and of the effect of disease mutations on WASH complex assembly and function
Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease, 1832(1), 160-173 (2013)
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