SAB4200365

Anti-PIWIL1 antibody, Mouse monoclonal

clone PIWIL1-2, purified from hybridoma cell culture

• 别名: Monoclonal Anti-PIWIL1 antibody produced in mouse, Monoclonal Anti-HIWI, Monoclonal Anti-MIWI, Monoclonal Anti-PIWI, Monoclonal Anti-piwi-like 1
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 品質等級: 200
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: PIWIL1-2, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~95 kDa
• 物種活性: human, mouse
• 濃度: ~1.0 mg/mL
• 技術: immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded mouse testis and biotin / ExtrAvidin^® Peroxidase staining system.; immunoprecipitation (IP): suitable; western blot: 2.5-5.0 μg/mL using whole cell extracts of HEK-293T cells over-expressing PIWIL1.
• 同型: IgG2b
• UniProt登錄號: Q96J94
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PIWIL1(9271) ; mouse ... Piwil1(57749)

说明

一般說明

Monoclonal Anti-PIWIL1 (mouse IgG2b isotype) is derived from the hybridoma piwi-like protein 1 (PIWIL1-2) produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to an internal region of human PIWIL1 conjugated to KLH. PIWIL1 is a member of Piwi subfamily. It is expressed mostly to the germline. The encoded protein consists of the PAZ domain and the PIWI domain. The PIWIL1 gene is located on human chromosome 12q24.33.

免疫原

synthetic peptide corresponding to an internal region of human PIWIL1, conjugated to KLH. The corresponding sequence differs by one amino acid in mouse PIWIL1.

應用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

Monoclonal Anti-PIWIL1 antibody produced in mouse has been used in:

• immunofluorescence
• immunoprecipitation
• flow cytometry analysis29}
• immunoblotting
• immunohistochemistry

生化／生理作用

Piwi proteins are implicated in epigenetic control of gene expression, transposon silencing and translation regulation. It plays vital roles in the germline development and gametogenesis of many metazoan species.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

法律資訊

ExtrAvidin is a registered trademark of Merck KGaA, Darmstadt, Germany

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable