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Merck
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主要文件

B0529

Sigma-Aldrich

Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat

affinity isolated antibody, buffered aqueous solution

别名:

Goat anti-Mouse IgG

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.46

生物源

goat

品質等級

共軛

biotin conjugate

抗體表格

affinity isolated antibody

抗體產品種類

secondary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

mouse

技術

direct ELISA: 1:200,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300
western blot: 1:300,000-1:600,000 using using Hela cells extract

儲存溫度

−20°C

目標翻譯後修改

unmodified

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一般說明

Binds all mouse Igs.
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. Each immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Mouse consists of five immunoglobulin classes- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.

應用

Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat has been used in immunohistochemistry and immunofluorescence studies.
Anti-Mouse IgG (Fab specific)-Biotin antibody produced in goat is suitable for direct ELISA at a dilution of 1:200000 and for immunohistochemistry at a dilution of 1:300. The antibody was used to stain paraffin-embedded, rat skin, duodenum, esophagus and spleen sections at a dilution of 1:200. For immunofluorescence of Human Gingival Fibroblasts, a dilution of 1:200 was used. For immunoblotting experiments using mouse adipose tissue, antibody dilution of 1:30000 was used.

生化/生理作用

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections.
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

其他說明

Antibody adsorbed with human IgG and rat serum proteins.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

準備報告

Adsorbed to reduce background staining with human or rat samples.

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Dendrophthoe pentandra (L.) Miq extract effectively inhibits inflammation, proliferation and induces p53 expression on colitis-associated colon cancer
Endharti AT, et al.
BMC Complementary and Alternative Medicine, 16(1), 374-374 (2016)
The Laboratory Rat (1998)
Targeted disruption of fibronectin-integrin interactions in human gingival fibroblasts by the RI protease ofPorphyromonas gingivalis W50
Scragg MA, et al.
Infection and Immunity, 67(4), 1837-1843 (1999)
Margaret A Scragg et al.
Infection and immunity, 70(10), 5740-5750 (2002-09-14)
Porphyromonas gingivalis is an important pathogen associated with destructive periodontal disease and is able to invade the epithelial cell barrier. Its cysteine proteases are recognized as major virulence factors, and in this study, we examined the interaction of the arginine-specific
Molecular Genetics of Immunoglobulin, 17 (1987)

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