S1073

Anti-STUB1/CHIP antibody, mouse monoclonal

clone ST21.55, purified from hybridoma cell culture

• 别名: Anti-C terminus of HSC70-interacting protein (CHIP), Anti-HSPABP2, Anti-Heat shock protein A binding protein 2 (c-terminal), Anti-NY-CO-7, Anti-SDCCAG7, Anti-STIP1 homologous and box-containing protein 1 (STUB1), Anti-Serologically defined colon cancer antigen 7, Anti-UBOX1
• MDL號碼: MFCD02262541
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: mouse
• 共軛: unconjugated
• 抗體表格: purified from hybridoma cell culture
• 抗體產品種類: primary antibodies
• 無性繁殖: ST21.55, monoclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~35 kDa
• 物種活性: rat, human, bovine
• 濃度: ~1.0 mg/mL
• 技術: indirect ELISA: suitable; western blot: 2.5-5 μg/mL using HeLa total cell extract.
• 同型: IgG2b
• UniProt登錄號: Q9UNE7
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: bovine ... STUB1(504565) ; human ... STUB1(10273) ; mouse ... Stub1(56424) ; rat ... Stub1(287155)

说明

一般說明

Monoclonal Anti-STUB1/CHIP (mouse IgG2b isotype) is derived from the hybridoma ST21.55 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP. STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) consists of three functional domains: a tetratricopeptide repeat (TRP) at the amino terminus, a U- box domain at the C-terminus, and a highly charged region separating the two.

STUB1 (STIP1 homology and U-box containing protein 1) or CHIP (carboxyl terminus of Hsc70- interacting protein) is an E3 ubiquitin ligase, (2) that is located on human chromosome 16p13.3.

免疫原

synthetic peptide corresponding to amino acids 30-45 of human STUB1/CHIP.

應用

Anti-STUB1/CHIP antibody, mouse monoclonal has been used in immunoblotting and enzyme-linked immunosorbent assay (ELISA).

生化／生理作用

In lens epithelial cells, knocking down CHIP (carboxyl terminus of Hsc70- interacting protein) decreased the reaction of heat shock and potential of protein quality control. It plays a major role in neurodegeneration and also controls autophagic flux. It can deactivate NF-κB signaling and damage the capability of migration and invasion in gastric cancer cells.

STUB1/CHIP (carboxyl terminus of Hsc70-interacting protein) is such a cofactor, which interacts, among others, with Hsc70 and acts as a U-box-dependent E3 ubiquitin ligase. STUB1/CHIP can also act as a direct chaperone of p53, both under physiological and stress conditions.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)