S0692

Spectinomycin

Ready Made Solution, 100 mg/mL in DMSO/H₂O, 1:1

• 别名: Actinospectacin
• MDL號碼: MFCD04041129
• 分類程式碼代碼: 51101500
• NACRES: NA.85

属性

• 生物源: Streptomyces sp.
• 品質等級: 200
• 形狀: solution
• 儲存條件: (Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage.Store under inert gas.)
• 濃度: 100 mg/mL in DMSO/H₂O, 1:1
• 顏色: colorless
• 溶解度: DMSO/H₂O, 1:1: soluble 100 mg/mL
• 抗生素活性譜: Gram-negative bacteria; Gram-positive bacteria; mycobacteria
• 作用方式: protein synthesis | interferes
• 運輸包裝: wet ice
• 儲存溫度: −20°C

说明

一般說明

Chemical structure: aminoglycoside

應用

Spectinomycin is an aminocyclitol antibiotic derived from Streptomyces spectabilis. It has been shown to have a wide variety of uses, including treating acute gonorrheal urethritis and cervictis, to mark cell layers to monitor cell fate during leaf development, as a selection marker in plant related transformation systems for plant cells containing the marker gene Spcr, to study respiratory tract infections of cattle caused by Pasteurella multocida and Mannheimia haemolytica, and to generate plants deficient for the plastid-encoded RNA polymerase on MS-agar media. Spectinomycin also has use in amplification of low copy number plasmid carrying replicons. It is recommended for use in cell culture applications at 7.5-20 mg/L.

生化／生理作用

作用方式：通过干扰肽基 tRNA 的转位抑制蛋白质合成（延伸）。
抗菌谱：革兰氏阴性和革兰氏阳性菌（仅包括淋球菌）。
抗性机理：rpsE（核糖体蛋白 S5 的基因）的突变可阻止壮观霉素的结合。

注意

Solutions can be stored at 2-8°C for several weeks or at -20°C for more extended periods.

準備報告

Stock solutions should be prepared at 10 mg/mL in water and 0.2 μm filtered.

其他說明

Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. Store under inert gas

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 2
• 閃點（°F）: 201.0 °F - closed cup
• 閃點（°C）: 93.9 °C - closed cup
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)