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一般說明
D-核酮糖1,5-二磷酸羧化酶(RUBISCO)占菠菜叶可溶蛋白总量的50%。
以一种557 kDa hexadecamer存在，它由8个分子量约为56 kDa的重链以及8个分子量约为14 kDa的轻链组成。每个分子包含一个镁离子。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
最适pH：~7.9。
CO2的KM：~0.45 mM。
核糖二磷酸在浓度超过0.7 mM时具有抑制作用。正磷酸和硫酸铵是竞争性抑制剂。3-磷酸甘油酸是一种非竞争性抑制剂。
應用
菠菜D-核酮糖1,5-二磷酸羧化酶已用于：
- 作为胃蛋白酶消化研究的测试蛋白
- 检测其作为无害蛋白样品，对人肠上皮细胞系的影响
- 和脱落酸的等温滴定量热法(ITC)和放射性标记结合检测
生化／生理作用
D-核酮糖1,5-二磷酸羧化酶（RUBISCO，核酮糖二磷酸羧化酶）的激活依赖Rubisco激活酶和分子伴侣。它参与植物光吸收事件，可催化核酮糖1,5-二磷酸的羧化和加氧。Rubisco酶的羧化活性受脱落酸抑制。
單位定義
在pH 7.8、25°C条件下，一单位可在每分钟将1.0 μmole的D-RuDP和CO2转化为2.0 μmole的D-3-磷酸甘油酸酯。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 1
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
Yuji Suzuki et al.
Journal of experimental botany, 64(4), 1145-1152 (2013-01-26)
Rubisco gene expression was examined in detail in rice (Oryza sativa L.) leaves at different positions, i.e. expanding, mature, and senescent leaves. Rubisco small subunit (RBCS) synthesis and RBCS mRNA levels were maximal in expanding leaves and gradually became lower
Romain A Studer et al.
Proceedings of the National Academy of Sciences of the United States of America, 111(6), 2223-2228 (2014-01-29)
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