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一般說明
研究领域：细胞信号传导
磷酸甘油酸激酶（PGK）是一种糖酵解酶，可从多种生物体中分离获得。这种典型的铰链弯曲单体酶在三域生物中都很保守。这种酶由单体折叠多肽链构成，可分成两个几乎相同的结构域，每个结构域由两个螺旋链（螺旋链7和14）连接并由一个深裂隙分离。这种排列使该酶具有独特的双叶结构。
磷酸甘油酸激酶（PGK）是一种糖酵解酶，可从多种生物体中分离获得。这种典型的铰链弯曲单体酶在三域生物中都很保守。这种酶由单体折叠多肽链构成，可分成两个几乎相同的结构域，每个结构域由两个螺旋链（螺旋链7和14）连接并由一个深裂隙分离。这种排列使该酶具有独特的双叶结构。
應用
来自面包酵母的′3-磷酸甘油酸磷酸激酶（酿酒酵母）已用于：
- 研究低分子量GTP结合蛋白和甘油醛-3-磷酸脱氢酶的抑制机制
- 偶联法测定纯化兔骨骼肌腺嘌呤二核苷酸（NAD+）-依赖性甘油醛-3-磷酸脱氢酶（GAPDH）的反向活性
- 测定甘油醛-3-磷酸脱氢酶
生化／生理作用
3-磷酸甘油酸磷酸激酶催化磷酸基团从1,3-二磷酸甘油酸到ADP的可逆转移，以生成ATP和3-磷酸甘油酸。 3-磷酸甘油酸磷酸激酶的活性对糖酵解和糖异生作用是必需的。磷酸甘油酸激酶（PGK）通过催化两个产生ATP反应之一的糖酵解途径中发挥着重要的作用。它将1,3-二磷酸甘油酸（1,3BPGA）转化为3-磷酸甘油酸（3PGA）。此外，它还通过催化对峙反应生成1,3BPGA和二磷酸腺苷（ADP）参与糖异生过程。由于其参与除能量代谢之外的许多过程，如发病机制、与核酸的相互作用、肿瘤发生和发展、细胞死亡及病毒复制等，PGK还被称为兼职蛋白质。
單位定義
一个单元将在pH6.9,25℃下每分钟将1.0μmol的1,3-二磷酸甘油酸转化为3-磷酸甘油酸。
外觀
3.0 M (NH4)2SO4和 0.04 M 焦磷酸四钠溶液 (pH 8.0) 中的晶体混悬液
分析報告
通过三氯乙酸(TCA)-双缩脲法测定的蛋白质。
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产品编号
说明
价格
訊號詞
Warning
危險聲明
危險分類
Eye Irrit. 2
儲存類別代碼
12 - Non Combustible Liquids
水污染物質分類（WGK）
WGK 2
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
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Instructions for working with enzymes supplied as ammonium sulfate suspensions
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