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Merck
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主要文件

PP0540

Sigma-Aldrich

糖谱 β-清除套件

Sufficient for 24 samples

别名:

β-Elimination Glycan Kit, Glycan Removal Kit

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About This Item

分類程式碼代碼:
12352200
NACRES:
NA.28

共軛

(O-linked)

儲存溫度

room temp

應用

GlycoProfile β-Elimination kit含有一种全新的非还原性试剂,可用于高效特异地去除糖蛋白中的O-连接的糖类,而对蛋白或多糖几乎不会造成破坏。因此,可以使用一些其他的下游蛋白质组学及多糖组学分析方法。

數量

该试剂盒包含可用于对多达24个样品(200 uL的1-10 ug/uL糖蛋白样品)进行O-去糖基化所需的试剂及配件。

法律資訊

GlycoProfile is a trademark of Sigma-Aldrich Co. LLC

仅试剂盒组分

产品编号
说明

  • Sodium hydroxide solution, 5.0 M 60 μL

  • Microcon centrifugal filter unit, YM-10 membrane, NMWCO 10 kDa 24 ea

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • Centrifugal Filter Unit

象形圖

FlameCorrosion

訊號詞

Danger

危險聲明

危險分類

Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1A

儲存類別代碼

3 - Flammable liquids

閃點(°F)

82.0 °F - closed cup

閃點(°C)

27.79 °C - closed cup


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Prediction of glycoprotein secondary structure using ATR-FTIR
Lewis, S.P., et al.
Vibrational Spectroscopy, 69, 21-29 (2013)

商品

Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays a major role in regulatory biology (as evident with studies around O-GlcNac and RNAi knockdowns of glycosyltransferases) the development of methods for the study of O-glycosylation is under represented compared to N-glycosylation. The under representation is primarily due to the difficulty in removing O-glycans while keeping both the protein and glycans intact. In contrast to N-glycosylation, there is no single enzyme capable of complete O-deglycosylation, so chemical methods must be employed.

Our's GlycoProfile Beta-Elimination Kit allows researchers to perform complete glycoproteomics research by preserving both the O-glycans and protein, specifically remove o-glycans, label o-glycans prior to analysis, have confidence in uniformity of procedure. While it is known that O-glycosylation plays a major role in regulatory biology (as evident with studies around O-GlcNac and RNAi knockdowns of glycosyltransferases) the development of methods for the study of O-glycosylation is under represented compared to N-glycosylation. The under representation is primarily due to the difficulty in removing O-glycans while keeping both the protein and glycans intact. In contrast to N-glycosylation, there is no single enzyme capable of complete O-deglycosylation, so chemical methods must be employed.

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