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一般說明
聚(脱氧鸟嘌呤-脱氧胞苷)酸(聚(dG-dC))经历了盐依赖性构象转变。在高盐下,以左旋双螺旋存在,而在低盐下则显示出B-DNA构象。然而,在固定化时聚(dG-dC)呈现DNA形式。
應用
聚(脱氧鸟嘌呤-脱氧胞苷)酸钠盐已用于:
- 转录因子IIIB复合物的电泳迁移率变动分析
- 作为用于圆二色(CD)光谱研究的合成共聚物
- 作为液体闪烁计数的阳性对照
聚(脱氧鸟嘌呤-脱氧胞苷)酸(聚(dG-dC)•聚(dG-dC))是一种交替共聚物、自身互补的双链DNA模型,用于DNA结构动力学和药物相互作用的构象研究。
其他說明
交替共聚物;自我互补,形成双链复合物
單位定義
一个酶活性单位是指在pH 7.0(1cm光程)条件下,在1.0 ml 20 mM磷酸钠/100 mM NaCl中产生1.0的A260 所需的酶量。
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Genomic DNA methylation decreases in response to moderate folate depletion in elderly women-
American Journal of Clinical Nutrition, 72(4), 998-1003 (2000)
Salt-dependent dynamic structure of poly (dG-dC) textperiodcentered poly (dG-dC)
Nature, 288(5789), 413-413 (1980)
TFIIIB subunit locations on U6 gene promoter DNA mapped by site-specific protein-DNA photo-cross-linking
Febs Letters, 590(10), 1488-1497 (2016)
Poly (dG)-poly (dC) DNA appears shorter than poly (dA)-poly (dT) and possibly adopts an A-related conformation on a mica surface under ambient conditions
Febs Letters, 581(30), 5843-5846 (2007)
Analytical chemistry, 77(5), 1483-1487 (2005-03-01)
We report here a novel, time-resolved, lanthanide-based energy-transfer assay utilizing nonoverlapping acceptor fluorophores, which have their absorption energetically at a higher level than the emittive transitions of the donor. The technique was studied by comparing a series of nonoverlapping acceptors
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