P7624

Anti-Profilin 1 (C-terminal) 兔抗

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 别名: Anti-PFN1
• MDL號碼: MFCD09265368
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~15 kDa
• 物種活性: mouse, human, rat
• 濃度: ~1 mg/mL
• 技術: immunoprecipitation (IP): 5-10 μg using mouse NIH3T3 cell lysates; indirect immunofluorescence: 10-20 μg/mL using rat NRK cells; western blot (chemiluminescent): 1-2 μg/mL using whole extract of human HeLa cells
• UniProt登錄號: P15735
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... PFN1(5216) ; mouse ... Pfn1(18643) ; rat ... Pfn1(64303)

说明

一般說明

Profilin 1 is a ubiquitous actin monomer-binding protein. Profilin 1 is the most ubiquitous and abundant and is highly expressed throughout development and adulthood in most tissues including brain.

免疫原

synthetic peptide corresponding to amino acid residues 128-140 of human profilin 1, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

應用

Anti-Profilin 1 (C-terminal) antibody produced in rabbit has been used in:

• immunoblotting
• immunostaining
• immunoprecipitation
• immunofluorescence
• profilin enzyme-linked immunosorbent assay (ELISA) analysis

生化／生理作用

Profilin 1 is involved in actin polymerization in response to extracellular signals. Profilins were shown to be important for normal cell proliferation, differentiation and motility. Deletion of profilin 1 gene leads to an embryonic lethal phenotype. Profilin 1 is a potent regulator of actin filament dynamics. Although profilin 1 prevents spontaneous actin polymerization by complexing with unpolymerized actin in vivo, actin-profilin complexes can be added to free barbed ends, thereby stimulating actin polymerization. Profilin 1 was suggested to act as a tumor suppressor protein based on its reduced expression in several types of invasive cancers and its ability to suppress tumorigenicity when over-expressed in breast cancer cells. Deletion of profilin 1 is associated with Miller-Dieker syndrome.

標靶描述

Profilin 1 is a ubiquitous actin monomer-binding proteininvolved in actin polymerization in response toextracellular signals.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)