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生物源
mouse
品質等級
共軛
unconjugated
抗體表格
purified immunoglobulin
抗體產品種類
primary antibodies
無性繁殖
35-206, monoclonal
形狀
buffered aqueous solution
物種活性
human, rat, mouse
濃度
~2 mg/mL
技術
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.04-0.08 μg/mL using whole extract of HeLa cell line
同型
IgG2b
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
human ... PLK1(5347)
mouse ... Plk1(18817)
rat ... Plk1(25515)
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一般說明
Monoclonal Anti- hPlk1 (Polo Like Kinase 1) (mouse IgG2b isotype) is derived from the 35-206 hybridoma produced by the fusion of mouse myeloma cells (PAI) and splenocytes from BALB/c mice immunized with human full length Plk1. Polo-like kinases (Plks) are a family of serine/threonine kinases. The Plk family is structurally and functionally related to the polo gene in Drosophila. Plk1 localizes during mitosis in centrosomes, kinetochores and in the central spindle.
免疫原
human full length Plk1.
應用
Monoclonal Anti-hPlk1 (Polo Like Kinase 1) antibody produced in mouse has been used in:
- enzyme linked immunosorbent assay (ELISA)
- immunoblotting
- immunoprecipitation
- immunocytochemistry
- immunofluorescence staining
生化／生理作用
PLK1 is a serine-threonine kinase that is expressed in mitotic cells and cancers. This kinase regulates mitotic entry by modulating cdc2/cyclin B and is also involved in Nlp-mediated nucleation of microtubules .
Polo Like Kinase 1 (Plk1) has a role in tumor progression. Plk1 is a target of the DNA damage checkpoint on the basis that its activity is inhibited by DNA damage in G2 and mitosis. Moreover, Plk1 was found to bind to the sequence-specific DNA binding domain of p53. This interaction between p53 and Plk1 inhibits the transcriptional activation and the proapoptotic functions of p53.
外觀
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Phosphorylation of mitotic kinesin-like protein 2 by polo-like kinase 1 is required for cytokinesis
Neef R, et al.
The Journal of cell biology, 162(5), 863-876 (2003)
Polo-like kinase 1 (Plk1) inhibits p53 function by physical interaction and phosphorylation
Ando K, et al.
The Journal of biological chemistry, 279(24), 25549-25561 (2004)
Association of spindle midzone particles with polo-like kinase 1 during meiosis in mouse and human oocytes
Otsuki J, et al.
Reproductive Biomedicine Online, 18(4), 522-528 (2009)
The crystal structure of the human polo-like kinase-1 polo box domain and its phospho-peptide complex
Cheng KY, et al.
The Embo Journal, 22(21), 5757-5768 (2003)
A K Roshak et al.
Cellular signalling, 12(6), 405-411 (2001-02-24)
Entry into mitosis by mammalian cells is triggered by the activation of the cdc2/cyclin B holoenzyme. This is accomplished by the specific dephosphorylation of key residues by the cdc25C phosphatase. The polo-like kinases are a family of serine/threonine kinases which
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