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P4664

Sigma-Aldrich

邻苯二胺 二盐酸盐

chromogenic, tablet

别名:

1,2-Phenylenediamine tablet, OPD, OPD Tablet

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About This Item

线性分子式:
C6H4(NH2)2·2HCl
CAS号:
615-28-1
分子量:
181.06
Beilstein:
3912045
MDL號碼:
MFCD00012966
分類程式碼代碼:
12352204
PubChem物質ID:
24898491
NACRES:
NA.83

product name

邻苯二胺 二盐酸盐, tablet, 15 mg substrate per tablet

品質等級

200

形狀

tablet

mp

258 °C (dec.) (lit.)

溶解度

water: 1 tablet/10 mL, clear, colorless

儲存溫度

2-8°C

SMILES 字串

Cl[H].Cl[H].Nc1ccccc1N

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI 密鑰

RIIWUGSYXOBDMC-UHFFFAOYSA-N

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相关类别

一般說明

邻苯二胺二盐酸盐（OPD）是一种显色底物，因为利用辣根过氧化物酶缀合物，因此可用于酶联免疫吸附试验（ELISA）程序。底物产生可溶的最终产物，其颜色为橙棕色，并且可以在450 m下通过分光光度计读取。可以用3N HCl或3M H2SO4终止OPD反应，并在492 nm处读取。

應用

邻苯二胺盐酸盐已用于：
  • 作为传统捕获底物，用于重组血凝素（rHA）蛋白的酶联免疫吸附法检测（ELISA）
  • 作为底物，通过ELISA法测定严重急性呼吸综合征冠状病毒2（SARS-CoV-2）免疫球蛋白G（IgG）抗体
  • 作为特异性比色试剂，利用S蛋白的SARS-CoV-2抗原在ELISA方案中进行辣根过氧化物酶（HRP）活性评估

重構

将 1 片片剂溶于 0.05 M 磷酸盐-柠檬酸盐缓冲液 (pH 5.0) 中，得到所需浓度（通常 OPD 浓度为 使用 0.4 mg/mL）。在使用前即刻给每 100ml 底物缓冲溶液加入 40μl 新鲜的 30% 过氧化氢。片剂分别装在箔包中。

訊號詞

Warning

危險分類

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

dust mask type N95 (US), Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges

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商品

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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