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P5412

Sigma-Aldrich

邻苯二胺

peroxidase substrate, chromogenic, tablet

别名:

1,2-二氨基苯, 1,2-苯二胺, OPD

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About This Item

经验公式（希尔记法）:
C6H8N2
CAS号:
95-54-5
分子量:
108.14
Beilstein:
606074
EC號碼:
202-430-6
MDL號碼:
MFCD00007721
分類程式碼代碼:
12352204
PubChem物質ID:
24898638
NACRES:
NA.83

产品名称

邻苯二胺, tablet, 20 mg substrate per tablet

蒸汽密度

3.7 (vs air)

蒸汽壓力

0.01 mmHg ( 25 °C)

形狀

tablet

bp

256-258 °C

mp

100-102 °C

螢光

λex 337 nm; λem 417 nm (after derivatization)

儲存溫度

2-8°C

SMILES 字串

Nc1ccccc1N

InChI

1S/C6H8N2/c7-5-3-1-2-4-6(5)8/h1-4H,7-8H2

InChI 密鑰

GEYOCULIXLDCMW-UHFFFAOYSA-N

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應用

邻苯二甲胺 Free Base 是过氧化物酶底物，适用于ELISA程序。 底物产生可溶的最终产物，其颜色为橙棕色，并且可以在 450nm 下通过分光光度计读取。可以用 3N HCl 或 3M H2SO4 终止 OPD 反应，并在 492nm 处读取。
邻苯二胺用于检测转化生长因子（TGF-β） 和使用酶联免疫吸附测定（ELISA），测量抗cTfRMAb-ScFv融合蛋白。

生化／生理作用

邻苯二甲胺（o-PD）是一种有效底物，可用于分光光度法辣根过氧化物酶（HRP）介导的酶联免疫吸附测定（ELISA），产生2,3-二氨基吩嗪（DAP）作为o-PD氧化的产物。

重構

将 1 片片剂溶于 0.05 M 磷酸盐-柠檬酸盐缓冲液 (pH 5.0) 中，得到所需浓度（通常 OPD 浓度为 使用 0.4 mg/mL）。在使用前立即每 100ml 底物缓冲溶液中加入 40μl 新鲜的 30% 过氧化氢。片剂分别包装在箔包中。

訊號詞

Danger

危險分類

Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

儲存類別代碼

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

水污染物質分類（WGK）

WGK 3

閃點（°F）

276.8 °F - closed cup

閃點（°C）

136 °C - closed cup

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
SLCD0525
SLBX5630
SLBT7268
SLBP6518V
SLBN1715V

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Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrate systems produce an insoluble NBT diformazan end product that is blue to purple in color and can be observed visually.

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