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Merck
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主要文件

P3369

Sigma-Aldrich

Protamine sulfate

solid, 90.0-110.0% dry basis,≥92% (chromatography), suitable for transduction

别名:

鱼精蛋白硫酸盐 来源于鲑鱼精子, 硫酸鱼精蛋白

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.77

产品名称

硫酸鱼精蛋白, meets USP testing specifications

agency

meets USP testing specifications

品質等級

化驗

≥92% (chromatography)
90.0-110.0% dry basis

形狀

solid

分子量

Mr 5000-10000

技術

transduction: suitable

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

硫酸鱼精蛋白是阳离子多肽,从鲑鱼精子中分离得到,分子量5 kDa。

應用

硫酸鱼精蛋白用于:
  • 将慢病毒转导进人胚胎293T细胞
  • 将 shRNA转导入间充质干细胞/骨髓基质细胞(MSC)
  • 将 慢病毒Sca1-OCT4质粒转导入人脐血造血祖细胞CD34+ 细胞

生化/生理作用

碱性鱼精蛋白与带负电荷的肝素结合。在心血管外科手术中使用鱼精蛋白有时会引发过敏反应。
鱼精蛋白有助于使用阳离子脂质体直接转染微生物 细胞和人类细胞。鱼精蛋白能够有效中和肝素的抗凝作用。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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其他客户在看

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Protamine sulfate down-regulates thrombin generation by inhibiting factor V activation
Ainle FN, et al.
Blood, 114(8), 1658-1665 (2009)
Moosung Lee et al.
eLife, 9 (2020-12-18)
The immunological synapse (IS) is a cell-cell junction between a T cell and a professional antigen-presenting cell. Since the IS formation is a critical step for the initiation of an antigen-specific immune response, various live-cell imaging techniques, most of which
V Diness et al.
Thrombosis and haemostasis, 56(3), 318-322 (1986-12-15)
The neutralization of a low molecular weight heparin (LHN-1) and conventional heparin (CH) by protamine sulfate has been studied in vitro and in vivo. In vitro, the APTT activity of CH was completely neutralized in parallel with the anti-Xa activity.
Serious anaphylactic reactions due to protamine sulfate: a systematic literature review
Nybo M and Madsen JS
Basic and Clinical Pharmacology and Toxicology, 103(2), 192-196 (2008)
shRNA-mediated decreases in c-Met levels affect the differentiation potential of human mesenchymal stem cells and reduce their capacity for tissue repair
Rosova I, et al.
Tissue Engineering: Part A, 16(8), 2627-2639 (2010)

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