推荐产品
product name
4-硝基苯基 N-乙酰基-β-D-氨基葡萄糖, ≥99% (TLC)
化驗
≥99% (TLC)
形狀
powder
雜質
≤0.05% Free p-nitrophenol
溶解度
water: 5 mg/mL, clear, colorless to very faintly yellow
儲存溫度
−20°C
SMILES 字串
CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O
InChI
1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1
InChI 密鑰
OMRLTNCLYHKQCK-DHGKCCLASA-N
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應用
N-乙酰基-β-D-氨基葡糖苷酶（NAG）已用作：
- 检测N-乙酰基-β-D-氨基葡糖苷酶（NAG）的底物
- 作为底物研究几丁质酶ChiB1的活性
- 评估大鼠嗜碱性白血病(RBL)-2H3细胞的脱粒情况
生化／生理作用
4-硝基苯基N-乙酰基-β-D-氨基葡糖苷（NP-GlcNAc）是一种广泛使用的己糖苷酶底物。酶作用裂解糖苷键并形成4-酚盐（pNP），测量波长为405 nm。NP-GlcNAc还可结合二乙基氨基乙基-a-环糊精（DEn-CD）使用，用于b-N-乙酰氨基葡萄糖苷酶的快速、准确速率测定方法。环糊精衍生物作为添加剂，在酶的最佳pH值5附近将4-酚盐电离成黄色的4-硝基酚盐。
基底
N-乙酰-氨基葡糖苷酶的发色底物。
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
A O Ilesanmi
African journal of medicine and medical sciences, 26(1-2), 87-89 (1997-03-01)
Using a chromogenic substrate for the lysosomal enzyme, hexosaminidase to estimate cell numbers, a sensitive, simple, and non-radioactive procedure has been previously developed (1) in which microtiter reaction wells are directly scanned using a spectrophotometer. The applications of this method
Andrzej Temeriusz et al.
Carbohydrate research, 341(15), 2581-2590 (2006-09-05)
The X-ray diffraction analysis of N-o-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamine (1), N-m-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamines, N-p-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamines, and their N-acetyl derivatives was performed. The sugar moieties always adopt (4)C1 conformations, however, due to crystal packing forces they are always slightly distorted. It was found that except N-acetyl, N-m-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamine
Jin-Jin Xie et al.
International journal of biological macromolecules, 39(4-5), 159-164 (2006-07-04)
Chemical modification of p-chloromercuribenzoate (PCMB) on beta-N-acetyl-d-glucosaminidase (NAGase, EC 3.2.1.52) from green crab (Scylla serrata) has been studied. The results show that sulfhydryl group is essential for the activity of the enzyme. Inhibitory kinetics of the enzyme by mercuric chloride
J Kukidome et al.
The Tohoku journal of experimental medicine, 194(1), 23-34 (2001-09-15)
COLO 201, human colon adenocarcinoma cells were incubated with artificial primers, p-nitrophenyl-glycoside derivatives at 1.0 mmol (mM) in the medium containing 10% fetal bovine serum to detect sugar chain elongation. However, when p-nitrophenyl-beta-N-acetylglucosamine (beta-GlcNAc-PNP) was added, the medium changed color
K Masaki et al.
Journal of protein chemistry, 20(2), 107-113 (2001-09-21)
Bombyx mori lysozyme is 10 amino acids shorter than hen egg-white lysozyme, which is a typical c-type lysozyme. It was expressed by using the methylotrophic yeast Pichia pastoris. The thermal stability and the enzymatic activity of the Bombyx mori lysozyme
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