跳转至内容
Merck

N9376

Sigma-Aldrich

4-硝基苯基 N-乙酰基-β-D-氨基葡萄糖

chromogenic, ≥99% (TLC), powder

别名:

4-硝基苯基 2-乙酰胺基-2-脱氧--β-D-吡喃葡糖苷

登录查看公司和协议定价


About This Item

经验公式(希尔记法):
C14H18N2O8
CAS号:
分子量:
342.30
Beilstein:
96193
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
PubChem物質ID:
NACRES:
NA.32

product name

4-硝基苯基 N-乙酰基-β-D-氨基葡萄糖, ≥99% (TLC)

品質等級

化驗

≥99% (TLC)

形狀

powder

雜質

≤0.05% Free p-nitrophenol

溶解度

water: 5 mg/mL, clear, colorless to very faintly yellow

儲存溫度

−20°C

SMILES 字串

CC(=O)N[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1Oc2ccc(cc2)[N+]([O-])=O

InChI

1S/C14H18N2O8/c1-7(18)15-11-13(20)12(19)10(6-17)24-14(11)23-9-4-2-8(3-5-9)16(21)22/h2-5,10-14,17,19-20H,6H2,1H3,(H,15,18)/t10-,11-,12-,13-,14-/m1/s1

InChI 密鑰

OMRLTNCLYHKQCK-DHGKCCLASA-N

正在寻找类似产品? 访问 产品对比指南

應用

N-乙酰基-β-D-氨基葡糖苷酶(NAG)已用作:
  • 检测N-乙酰基-β-D-氨基葡糖苷酶(NAG)的底物
  • 作为底物研究几丁质酶ChiB1的活性
  • 评估大鼠嗜碱性白血病(RBL)-2H3细胞的脱粒情况

生化/生理作用

4-硝基苯基N-乙酰基-β-D-氨基葡糖苷(NP-GlcNAc)是一种广泛使用的己糖苷酶底物。酶作用裂解糖苷键并形成4-酚盐(pNP),测量波长为405 nm。NP-GlcNAc还可结合二乙基氨基乙基-a-环糊精(DEn-CD)使用,用于b-N-乙酰氨基葡萄糖苷酶的快速、准确速率测定方法。环糊精衍生物作为添加剂,在酶的最佳pH值5附近将4-酚盐电离成黄色的4-硝基酚盐。

基底

N-乙酰-氨基葡糖苷酶的发色底物。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

A O Ilesanmi
African journal of medicine and medical sciences, 26(1-2), 87-89 (1997-03-01)
Using a chromogenic substrate for the lysosomal enzyme, hexosaminidase to estimate cell numbers, a sensitive, simple, and non-radioactive procedure has been previously developed (1) in which microtiter reaction wells are directly scanned using a spectrophotometer. The applications of this method
Andrzej Temeriusz et al.
Carbohydrate research, 341(15), 2581-2590 (2006-09-05)
The X-ray diffraction analysis of N-o-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamine (1), N-m-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamines, N-p-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamines, and their N-acetyl derivatives was performed. The sugar moieties always adopt (4)C1 conformations, however, due to crystal packing forces they are always slightly distorted. It was found that except N-acetyl, N-m-nitrophenyl-2,3,4,6-tetra-O-acetyl-beta-D-glucopyranosylamine
Jin-Jin Xie et al.
International journal of biological macromolecules, 39(4-5), 159-164 (2006-07-04)
Chemical modification of p-chloromercuribenzoate (PCMB) on beta-N-acetyl-d-glucosaminidase (NAGase, EC 3.2.1.52) from green crab (Scylla serrata) has been studied. The results show that sulfhydryl group is essential for the activity of the enzyme. Inhibitory kinetics of the enzyme by mercuric chloride
J Kukidome et al.
The Tohoku journal of experimental medicine, 194(1), 23-34 (2001-09-15)
COLO 201, human colon adenocarcinoma cells were incubated with artificial primers, p-nitrophenyl-glycoside derivatives at 1.0 mmol (mM) in the medium containing 10% fetal bovine serum to detect sugar chain elongation. However, when p-nitrophenyl-beta-N-acetylglucosamine (beta-GlcNAc-PNP) was added, the medium changed color
K Masaki et al.
Journal of protein chemistry, 20(2), 107-113 (2001-09-21)
Bombyx mori lysozyme is 10 amino acids shorter than hen egg-white lysozyme, which is a typical c-type lysozyme. It was expressed by using the methylotrophic yeast Pichia pastoris. The thermal stability and the enzymatic activity of the Bombyx mori lysozyme

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门