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一般說明
硝酸还原酶(NAD [P] H)在多种生物中表达,包括细菌、真菌、藻类和高等植物,它们具有使用硝酸盐作为其唯一氮源的能力。实验研究表明,硝酸还原酶(NAD [P] H))的大小为180,000道尔顿。
生化/生理作用
硝酸还原酶(NAD [P] H)可催化硝酸盐还原成亚硝酸盐。此外,它还可催化NADPH-细胞色素c、FADH2-硝酸盐的还原。硝酸还原酶还具有降低的甲基紫精-硝酸盐还原酶活性。
注意
光线和湿度敏感
單位定義
在存在 β-NADPH,pH7.6,37℃条件下,一个单位每分钟可还原 1.0 μmole 的硝酸盐。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Molecular microbiology, 25(2), 205-210 (1997-07-01)
The FNR (fumarate and nitrate reductase regulation) protein of Escherichia coli is an oxygen-responsive transcriptional regulator required for the switch from aerobic to anaerobic metabolism. In the absence of oxygen, FNR changes from the inactive to the active state. The
Molecular microbiology, 24(5), 1049-1060 (1997-06-01)
The Escherichia coli NarX, NarQ, NarL and NarP proteins comprise a two-component regulatory system that controls the expression of many anaerobic electron-transport and fermentation-related genes in response to nitrate and nitrite. Either of the two sensor-transmitter proteins, NarX and NarQ
Cellular and molecular life sciences : CMLS, 58(2), 194-204 (2001-04-06)
Pyridine nucleotide-dependent nitrate reductases (NRs; EC 1.6.6.1-3) are molybdenum-containing enzymes found in eukaryotic organisms which assimilate nitrate. NR is a homodimer with an approximately 100 kDa polypeptide which folds into stable domains housing each of the enzyme's redox cofactors--FAD, heme-Fe
Purification and properties of assimilatory nitrate reductase [NAD(P)H] from Ankistrodesmus braunii.
European journal of biochemistry, 106(1), 249-256 (1980-05-01)
Assimilatory nitrate reductase [NAD(P)H] (EC 1.6.6.2) from Ankistrodesmus braunii has been purified to homogeneity by a simple procedure that utilizes as the main step affinity chromatography on Blue-Sepharose. The best enzyme preparation has a specific activity of 61.25 units/mg protein.
Molecular & general genetics : MGG, 228(3), 329-334 (1991-09-01)
Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific nitrate reductases. Genomic and cDNA clones of the NADH nitrate reductase have been sequenced. In this study, a genomic clone (pMJ4.1) of a second type of nitrate reductase was isolated from
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