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Merck

N7265

Sigma-Aldrich

硝酸还原酶 (NAD[P]H) 来源于黑曲霉

lyophilized powder, ≥300 units/g solid

别名:

NAD(P)H:硝酸氧化还原酶

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

品質等級

比活性

≥300 units/g solid

運輸包裝

wet ice

儲存溫度

−20°C

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一般說明

硝酸还原酶(NAD [P] H)在多种生物中表达,包括细菌、真菌、藻类和高等植物,它们具有使用硝酸盐作为其唯一氮源的能力。实验研究表明,硝酸还原酶(NAD [P] H))的大小为180,000道尔顿。

生化/生理作用

硝酸还原酶(NAD [P] H)可催化硝酸盐还原成亚硝酸盐。此外,它还可催化NADPH-细胞色素c、FADH2-硝酸盐的还原。硝酸还原酶还具有降低的甲基紫精-硝酸盐还原酶活性。

注意

光线和湿度敏感

單位定義

在存在 β-NADPH,pH7.6,37℃条件下,一个单位每分钟可还原 1.0 μmole 的硝酸盐。

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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G Unden et al.
Molecular microbiology, 25(2), 205-210 (1997-07-01)
The FNR (fumarate and nitrate reductase regulation) protein of Escherichia coli is an oxygen-responsive transcriptional regulator required for the switch from aerobic to anaerobic metabolism. In the absence of oxygen, FNR changes from the inactive to the active state. The
R C Chiang et al.
Molecular microbiology, 24(5), 1049-1060 (1997-06-01)
The Escherichia coli NarX, NarQ, NarL and NarP proteins comprise a two-component regulatory system that controls the expression of many anaerobic electron-transport and fermentation-related genes in response to nitrate and nitrite. Either of the two sensor-transmitter proteins, NarX and NarQ
W H Campbell
Cellular and molecular life sciences : CMLS, 58(2), 194-204 (2001-04-06)
Pyridine nucleotide-dependent nitrate reductases (NRs; EC 1.6.6.1-3) are molybdenum-containing enzymes found in eukaryotic organisms which assimilate nitrate. NR is a homodimer with an approximately 100 kDa polypeptide which folds into stable domains housing each of the enzyme's redox cofactors--FAD, heme-Fe
M A de la Rosa et al.
European journal of biochemistry, 106(1), 249-256 (1980-05-01)
Assimilatory nitrate reductase [NAD(P)H] (EC 1.6.6.2) from Ankistrodesmus braunii has been purified to homogeneity by a simple procedure that utilizes as the main step affinity chromatography on Blue-Sepharose. The best enzyme preparation has a specific activity of 61.25 units/mg protein.
J Miyazaki et al.
Molecular & general genetics : MGG, 228(3), 329-334 (1991-09-01)
Barley (Hordeum vulgare L.) has both NADH-specific and NAD(P)H-bispecific nitrate reductases. Genomic and cDNA clones of the NADH nitrate reductase have been sequenced. In this study, a genomic clone (pMJ4.1) of a second type of nitrate reductase was isolated from

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