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Merck

MTOXCE2C9

Sigma-Aldrich

CypExpress 2C9 Cytochrome P450 human

别名:

CPC9, CYP2C, CYP2C10, CYP2C9, CYPIIC9, P450 2C9, P450IIC9

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About This Item

EC號碼:
分類程式碼代碼:
41106200
NACRES:
NA.77

生物源

human

重組細胞

expressed in Pichia pastoris

形狀

dry powder

UniProt登錄號

應用

cell analysis

運輸包裝

dry ice

儲存溫度

−70°C

基因資訊

human ... CYP2C9(1559)

生化/生理作用

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. Sulfaphenazole, fluconazole, fluvoxamine and fluoxetine are inhibitors, whereas rifampin is an inducer of the enzyme. The isozyme CYP2C9 is a major pathway for the invivo conversion of Diclofenac to 4-Hydroxydiclofenac via hydroxylation.
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

特點和優勢

CypExpress 2C9 is a permeabilized and stabilized dried yeast powder preparation containing full length, unmodified, human CYP 2C9 and recombinant human NADPH oxidoreductase. Using proprietary processes, CypExpress are expressed in Pichia yeast. Following optimal expression, the cells are inactivated, permeabilized and dried to a fine powder. CypExpress retains the cellular mechanisms to provide the P450 enzymes with the energy and cofactors to continue to function for long experiments, and can generate larger amounts of metabolite than mammalian microsomes or other genetically engineered expression systems. It is designed for rapid screening of drug metabolites and for scaling up metabolite generation.

單位定義

A reaction mixture containing 500 μM diclofenac and 100 mg/mL of CypExpress 2C9 will produce 428 μM 4-hydroxydiclofenac in 2 hours at 30°C under the conditions described.

外觀

Light yellow, very fine powder

重構

Per Protocol/Variable

法律資訊

CypExpress is a trademark of Oxford Biomedical Research

相關產品

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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