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MTOX1012

Sigma-Aldrich

CAR Knockout HepaRG™ Cells

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

别名:

CAR knockout human hepatocyte cells, HepaRG CAR KO

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.81

product name

CAR Knockout HepaRG™ Cells, 1 vial

生物源

human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)

形狀

liquid

儲存溫度

−196°C

基因資訊

human ... ABCB1(1346307)

一般說明

HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG CAR KO cells express the major xenobiotic sensors (PXR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.

應用

See technical bulletin for detailed protocols

特點和優勢

HepaRG cells are the most metabolically active human hepatocyte cell line developed to date. These cells are suitable for a wide variety of studies for drug metabolism, CYP induction, metabolism-mediated toxicity, transporter, and hepatotoxicity.

  • Sigma′s HepaRG CAR Knockout (KO) allows conclusive identification of CYP2B6 induction through CAR activation.

Zinc finger nucleases (ZFN) mediated Knock-out of NR1I3 (CAR) gene.

  • The frame-shift mutation of NR1I3 gene was confirmed by fragment length analysis and DNA sequencing.
  • Loss of functionality was confirmed by loss of response to inducers of cytochrome P450 activity.

品質

Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation

法律資訊

These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
HepaRG is a trademark of BioPredic International company

免責聲明

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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Daochuan Li et al.
Biochemical pharmacology, 98(1), 190-202 (2015-08-16)
The constitutive androstane receptor (CAR) modulates the transcription of numerous genes involving drug metabolism, energy homeostasis, and cell proliferation. Most functions of CAR however were defined from animal studies. Given the known species difference of CAR and the significant cross-talk
Wenwei Lin et al.
Molecular pharmacology, 97(3), 180-190 (2019-12-29)
The xenobiotic receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) are activated by structurally diverse chemicals to regulate the expression of target genes, and they have overlapping regulation in terms of ligands and target genes. Receptor-selective agonists are

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