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用途
sufficient for 25 reactions
品質等級
品質
Not suitable for clinical diagnostic use.
包裝
pkg of 1 kit
儲存條件
dry at room temperature
技術
PCR: suitable
應用
detection
microbiology
儲存溫度
2-8°C
一般說明
引物/探针系统可检测支原体基因组中高度保守的23S rRNA操纵子编码区。该试剂盒具有高度特异性,不会检测出真核DNA。检测范围包括被鉴定为细胞培养污染物的大多数支原体物种(参见技术公报中的表2)。该试剂盒含有核苷酸dUTP而非dTTP,因此适用于UNG预处理。
该试剂盒的Mycoplasma Mix中含有 Taq 聚合酶。
應用
法律資訊
儲存類別代碼
10 - Combustible liquids
閃點(°F)
188.6 °F
閃點(°C)
87 °C
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Know when to use antibiotics to prevent contamination in cell culture and find related products at Sigma-Aldrich.com
Know when to use antibiotics to prevent contamination in cell culture and find related products at Sigma-Aldrich.com
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.
实验方案
Mycoplasma contamination of cell cultures is a serious issue impacting cell model validity. PCR testing for mycoplasma is an inexpensive, sensitive, and specific method for detecting contamination.
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