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Merck

MAK508

Sigma-Aldrich

Choline Assay Kit

sufficient for 100 colorimetric or fluorometric tests

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About This Item

分類程式碼代碼:
12161503
NACRES:
NA.84

品質等級

應用

food and beverages
pharmaceutical

檢測方法

colorimetric
fluorometric

相關疾病

anxiety; neurological disorders

運輸包裝

wet ice

儲存溫度

−20°C

一般說明

Choline and its metabolites play important roles in membrane structure integrity, cellular signaling and cholinergic neurotransmission. Aberrant regulation in choline metabolism has been associated with mental illness such as anxiety.

特點和優勢

  • Linear detection range: 1 to 100 µM for the colorimetric assay and 0.2 to 10 µM for the fluorometric assay.

適合性

The kit is suitable for the determination of choline in biological samples such as serum, plasma, urine, saliva, milk, tissue, and cell culture, as well as for studying the effects of drugs on choline metabolism.

原則

The Choline Assay Kit method provides a simple, direct and high-throughput assay for measuring choline in biological samples. In this assay, free choline is oxidized by choline oxidase to betaine and H2O2 which reacts with a specific dye to form a pink colored product. The color intensity at 570 nm or fluorescence intensity λEx = 530 nm/λEm = 585 nm is directly proportional to the choline concentration in the sample.

象形圖

Health hazard

訊號詞

Danger

危險聲明

危險分類

Aquatic Chronic 3 - Resp. Sens. 1

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3


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