用途
sufficient for 100 colorimetric or fluorometric tests
應用
cosmetics
food and beverages
pharmaceutical
檢測方法
colorimetric
fluorometric
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
磷脂是一种脂质，是构成细胞膜的重要组成部分，在信号转导中起着重要作用。大多数磷脂包含一分子甘油二酯，一分子磷酸基和一分子胆碱。
在该检测中,磷脂（如卵磷脂、溶血卵磷脂和鞘磷脂）会被酶促水解为胆碱，随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物，此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM，荧光法则为0.6 - 20 μM。
在该检测中,磷脂（如卵磷脂、溶血卵磷脂和鞘磷脂）会被酶促水解为胆碱，随后使用胆碱氧化酶和H2O2特异性染料进行测定。反应会产生比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物，此产物与样品中的磷脂浓度成正比。比色法的线性检测范围是3 - 200 μM，荧光法则为0.6 - 20 μM。
應用
磷脂测定试剂盒已用于生化分析 和磷脂定量。
特點和優勢
兼容高通量的操作系统。
適合性
适用于生物样品中的磷脂检测。
原則
在该检测中，磷脂（如卵磷脂、溶血卵磷脂和鞘磷脂）会被酶法水解以释放胆碱，而胆碱则可通过胆碱氧化酶和H2O2-特异性染料所测定。所得的比色(570 nm)/荧光(λex = 530/λem = 585 nm)产物与样品中的磷脂浓度成正比。对于比色测定，检测的线性范围是3 - 200 μM；对于荧光测定，检测的线性范围是0.6 - 20 μM。
訊號詞
Danger
危險聲明
危險分類
Aquatic Chronic 3 - Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
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