推荐产品
檢測方法
colorimetric
fluorometric
相關疾病
cancer; neurological disorders
儲存溫度
−20°C
一般說明
三磷酸腺苷(ATP)是一种仅在线粒体内形成的三磷酸核苷。这是一种高能分子,被称为所有生命系统的能量货币。包含在ATP的磷酸键中的化学能量驱动着大多数的细胞过程。诸如Leber遗传性视神经病变、Leigh综合征、神经病、共济失调以及色素性视网膜炎等遗传疾病都会影响线粒体内的ATP生成。
應用
ATP检测试剂盒已用于通过对样本中ATP的荧光检测而对ATP浓度进行量化。
適合性
适于测定不同样品中的ATP,如动物组织和细胞培养样品。
原則
ATP比色/荧光检测试剂盒是一种用于测定各种样品中ATP的灵敏检测(比色检测为2-10 nmole/孔而荧光检测为20-1,000 pmole/孔)。ATP的浓度是通过磷酸化甘油而测定,可产生与存在的ATP量成比例的比色(570 nm)或荧光(lex = 535/lem = 587 nm)产物。
其他說明
荧光检测比比色检测具有10-100倍更高的灵敏度。
仅试剂盒组分
产品编号
说明
- ATP Assay Buffer
- ATP Probe, in DMSO
- ATP Converter
- Developer Mix
- ATP Standard, 1.0 μmole
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點(°F)
188.6 °F
閃點(°C)
87 °C
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