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用途
sufficient for 100 colorimetric or fluorometric tests
檢測方法
colorimetric
fluorometric
儲存溫度
−20°C
一般說明
丙氨酸是一种非必需氨基酸,在肌肉组织和肝脏之间的葡萄糖-丙氨酸循环中起关键作用。在氨基酸降解组织(如肌肉)中,氨基通过转氨反应汇集为谷氨酸。谷氨酸的氨基通过丙氨酸氨基转移酶转移到丙酮酸,形成丙氨酸和 α-酮戊二酸。丙氨酸进入血液并转运至肝脏。该反应在肝脏中逆转,丙酮酸盐可用于糖异生形成葡萄糖 ,葡萄糖可通过循环系统返回至其他组织。丙氨酸水平升高与高血压、能量摄入、胆固醇水平和体重指数相关。
應用
丙氨酸检测试剂盒已用于测定细胞内的丙氨酸池。
適合性
适用于细胞和组织培养上清、尿液、血浆、血清等生物样本中的丙氨酸检测。
原則
在本试剂盒中,丙氨酸浓度由偶联酶分析法测定,其结果为比色法 (570 nm)/荧光法(λex = 535/λ em = 587 nm)乘积,与存在的丙氨酸成比例。本试剂盒的典型检测范围为 2-10 nmole(比色法)和 0.2-1 nmole(荧光测定法)。
訊號詞
Danger
危險聲明
危險分類
Resp. Sens. 1 - Skin Sens. 1
儲存類別代碼
10 - Combustible liquids
閃點(°F)
188.6 °F - closed cup
閃點(°C)
87 °C - closed cup
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