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Merck
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M4526

Sigma-Aldrich

Eagle最低必需培养基

Alpha Modification, with sodium bicarbonate, without L-glutamine, ribonucleosides and deoxyribonucleosides, liquid, sterile-filtered, suitable for cell culture

别名:

αMEM, MEM

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About This Item

分類程式碼代碼:
12352207

品質等級

400

無菌

sterile-filtered

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

Earle’s salts (5% CO2): yes
sodium pyruvate: yes
glucose: yes
L-glutamine: no
HEPES: no
Hanks’ salts (2% CO2): no
phenol red: yes
NaHCO3: yes
stable glutamine: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

最小必需培养基Eagle（MEM）是一种合成细胞培养基，由Harry Eagle开发。它有更高浓度的氨基酸，所以培养基更接近培养哺乳动物细胞的蛋白质组成。在含有Hanks或Earle盐的配方中选择补充非必需氨基酸，可增强该培养基的用途。Earle平衡盐的MEM修饰也称为αMEM，含有非必需氨基酸、丙酮酸钠和其他维生素。
这是所提供的MEM配方中最丰富的变体。它含有所有21种正常氨基酸，其中一些的浓度有所增加。此外，它还含有5种额外的维生素。

應用

最小必需培养基Eagle已用于维持：
  • 小鼠未成熟的卵母细胞络合物
  • 人类间充质干细胞（MSC）
  • 小鼠成骨前细胞系

重構

添加 0.292g/L L-谷氨酰胺。

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類（WGK）

WGK 1

閃點（°F）

Not applicable

閃點（°C）

Not applicable

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