形狀
buffered aqueous glycerol solution
比活性
600-1000 units/mg protein (biuret)
正離子痕跡
NH4+: ≤10 μg/mg protein
異物活動
Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%
運輸包裝
wet ice
儲存溫度
2-8°C
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一般說明
线粒体同工酶
苹果酸脱氢酶是线粒体同工酶,也是三羧酸循环中的重要催化剂。
應用
采用苹果酸脱氢酶对银杏提取物对寄主钉螺的软体动物的杀灭作用进行了比较研究。 它还被用于研究早期饲养限制对肉鸡代谢程序和补偿性生长的影响。
生化/生理作用
真核细胞含有两种不同的苹果酸脱氢酶同工酶:线粒体同工酶(m-MDH)和可溶性或细胞质同工酶(s-MDH)。该产品由线粒体同工酶形式组成。
该酶可催化以下反应:草酰乙酸+β-NADH→ L-苹果酸+β-NAD
该酶可催化以下反应:草酰乙酸+β-NADH→ L-苹果酸+β-NAD
通过在25℃下用5′-磷酸吡哆醛处理可以可逆地对苹果酸脱氢酶进行灭活。
單位定義
在pH7.5,25℃条件下,一个单位每分钟可将1.0 μmole的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。
外觀
溶于含50%甘油的0.05M磷酸钾缓冲液(pH7.5)中
儲存類別代碼
10 - Combustible liquids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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