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Key Documents

L9516

Sigma-Aldrich

Lipopolysaccharides (rough strains) from Salmonella enterica serotype typhimurium SL1181

别名:

LPS

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About This Item

MDL號碼:
分類程式碼代碼:
12352201

生物源

Salmonella enterica (Serotype typhimurium SL1181)

形狀

lyophilized powder

雜質

≤5% Protein (Lowry)

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

This product is phenol:chloroform:petroleum ether extracted from Salmonella typhimurium strain SL1181. The source strain is from a private collection. This LPS has been used as a negative control for antibody binding to LPS.

生化/生理作用

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

準備報告

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

其他說明

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

儲存類別代碼

13 - Non Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Journal of Alzheimer's disease : JAD, 60(1), 23-42 (2017-08-05)
The mechanisms of neurodegeneration in Alzheimer's disease (AD) remain under investigation. Alterations in the blood-brain barrier facilitate exchange of inflammatory mediators and immune cells between the brain and the periphery in AD. Here, we report analysis of phenotype and functions
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