推荐产品
生物源
sheep
品質等級
共軛
rhodamine conjugate
抗體表格
purified antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
lyophilized (clear, red solution after reconstitution)
包裝
pkg of 200 μg
製造商/商標名
Roche
同型
IgG
儲存溫度
2-8°C
一般說明
地高辛是一种半抗原,可用于核酸标记和检测系统。本产品包含多克隆抗地高辛抗体的Fab片段,与罗丹明偶联。
特異性
绵羊的多克隆抗体显示出与地高辛和地高辛100%的反应性,但与其他类固醇如人雌激素(如雌二醇)或雄激素(如睾酮)没有交叉反应性。
應用
使用抗地高辛-罗丹明Fab片段检测地高辛标记的化合物:
- 在糖缀合物研究中使用地高辛标记糖
- 在原位杂交中使用荧光
- 免疫组织细胞化学
- 原位杂交
- 脱氧核糖核酸纤维分析
準備報告
地高辛免疫后,用离子交换色谱法纯化绵羊IgG,用免疫吸附法分离特异性IgG。木瓜蛋白酶消化获得的Fab片段通过凝胶过滤纯化,与特定标记缀合,并稳定在缓冲液中。
工作浓度:共轭物的工作浓度取决于应用和底物。以下浓度应作为指导:糖缀合物中地高辛标记糖的检测:20至50 & # 956;g/ml
工作溶液:PBS,0.5%牛血清白蛋白,pH 7.4。
1%封闭剂(w/v)、1-5%热灭活胎牛血清(v/v)或绵羊正常血清可用于降低非特异性结合。此外,可将酸碱度提高至8.5至9.0。
储存条件(工作方案):必须制备新鲜物料!
工作浓度:共轭物的工作浓度取决于应用和底物。以下浓度应作为指导:糖缀合物中地高辛标记糖的检测:20至50 & # 956;g/ml
- 荧光原位杂交:1-20 & # 956;g/ml
- 免疫组织细胞化学:20-50 & # 956;g/ml
- 原位杂交:20-50 & # 956;g/ml
工作溶液:PBS,0.5%牛血清白蛋白,pH 7.4。
1%封闭剂(w/v)、1-5%热灭活胎牛血清(v/v)或绵羊正常血清可用于降低非特异性结合。此外,可将酸碱度提高至8.5至9.0。
储存条件(工作方案):必须制备新鲜物料!
重構
加入1ml双蒸水至终浓度为200μg/ml。
其他說明
仅用于生命科学研究。不可用于诊断。
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訊號詞
Warning
危險聲明
危險分類
Aquatic Chronic 3 - Skin Sens. 1
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 2
閃點(°F)
does not flash
閃點(°C)
does not flash
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