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Merck

11207750910

Roche

抗地高辛-罗丹明,Fab片段

from sheep

别名:

地高辛配基, 抗-地高辛配基抗体

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About This Item

分類程式碼代碼:
12352203

生物源

sheep

品質等級

共軛

rhodamine conjugate

抗體表格

purified antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

lyophilized (clear, red solution after reconstitution)

包裝

pkg of 200 μg

製造商/商標名

Roche

同型

IgG

儲存溫度

2-8°C

一般說明

地高辛是一种半抗原,可用于核酸标记和检测系统。本产品包含多克隆抗地高辛抗体的Fab片段,与罗丹明偶联。

特異性

绵羊的多克隆抗体显示出与地高辛和地高辛100%的反应性,但与其他类固醇如人雌激素(如雌二醇)或雄激素(如睾酮)没有交叉反应性。

應用

使用抗地高辛-罗丹明Fab片段检测地高辛标记的化合物:
  • 在糖缀合物研究中使用地高辛标记糖
  • 原位杂交中使用荧光
  • 免疫组织细胞化学
  • 原位杂交
  • 脱氧核糖核酸纤维分析

準備報告

地高辛免疫后,用离子交换色谱法纯化绵羊IgG,用免疫吸附法分离特异性IgG。木瓜蛋白酶消化获得的Fab片段通过凝胶过滤纯化,与特定标记缀合,并稳定在缓冲液中。

工作浓度:共轭物的工作浓度取决于应用和底物。以下浓度应作为指导:糖缀合物中地高辛标记糖的检测:20至50 & # 956;g/ml
  • 荧光原位杂交:1-20 & # 956;g/ml
  • 免疫组织细胞化学:20-50 & # 956;g/ml
  • 原位杂交:20-50 & # 956;g/ml

工作溶液:PBS,0.5%牛血清白蛋白,pH 7.4。
1%封闭剂(w/v)、1-5%热灭活胎牛血清(v/v)或绵羊正常血清可用于降低非特异性结合。此外,可将酸碱度提高至8.5至9.0。

储存条件(工作方案):必须制备新鲜物料!

重構

加入1ml双蒸水至终浓度为200μg/ml。

其他說明

仅用于生命科学研究。不可用于诊断。

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象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Aquatic Chronic 3 - Skin Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 2

閃點(°F)

does not flash

閃點(°C)

does not flash


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Spencer W Luebben et al.
Nucleic acids research, 41(22), 10283-10297 (2013-09-06)
HELQ is a superfamily 2 DNA helicase found in archaea and metazoans. It has been implicated in processing stalled replication forks and in repairing DNA double-strand breaks and inter-strand crosslinks. Though previous studies have suggested the possibility that HELQ is
Galina Pendinen et al.
Genome, 51(9), 714-720 (2008-09-06)
Thirty-six percent of the wild potato (Solanum L. section Petota Dumort.) species are polyploid, and about half of the polyploids are tetraploid species (2n = 4x = 48). Determination of the type of polyploidy and development of the genome concept
Olga G Silkova et al.
Plants (Basel, Switzerland), 10(10) (2021-10-24)
To date, few data have been accumulated on the contribution of meiotic restitution to the formation of Triticum aestivum hybrid karyotypes. In this study, based on FISH and C-banding, karyotype reorganization was observed in three groups of F5 wheat-rye hybrids
Shin-Ichiro Takebayashi et al.
Epigenetics & chromatin, 6(1), 42-42 (2013-12-18)
Cellular differentiation and reprogramming are accompanied by changes in replication timing and 3D organization of large-scale (400 to 800 Kb) chromosomal domains ('replication domains'), but few gene products have been identified whose disruption affects these properties. Here we show that
Xiaochun Wu et al.
The FEBS journal, 285(21), 3945-3958 (2018-09-07)
The krüppel-like factors (KLFs) are a family of transcription factor proteins that regulate a wide range of biological processes. In an RNAi-based screening experiment, we identified dendritic arbor reduction 1 (Dar1), which is a KLF member in Drosophila, that inhibited

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Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.

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