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一般說明
本品从 O55:B5 血清型大肠杆菌中提取,经层析纯化。来源菌株为 CDC 1644-70。脂质 A 部分已通过碱水解部分脱脂。去除类脂 A 的脂肪酸部分可产生一种去毒 LPS,其内毒素级别比母体 LPS 低 10,000 倍。
應用
脂多糖(LPS)是革兰氏阴性细菌细胞壁的特征组分。脂多糖及其脂质 A 部分通过 Toll 样受体 4(TLR4)刺激先天免疫系统的细胞,Toll 样受体 4是 Toll 样受体蛋白家族的成员,其识别常见的病原体相关分子模式(PAMP)。
生化/生理作用
脂多糖(LPS)位于膜的外层,并且在非包封的菌株中,其暴露于细胞表面。它们有助于外膜的完整性,并可保护细胞免受胆汁盐和亲脂性抗生素的作用。
準備報告
色谱纯化;碱水解脱脂
该产品可溶于水(5mg / ml)或细胞培养基(1mg / ml),产生浑浊的淡黄色溶液。当涡旋并升温至70-80 oC后,在盐水中可获得更高浓度的溶液(20mg/ml),但仍然是浑浊的溶液。脂多糖是一种在各种溶剂中都形成胶束的分子。在水和磷酸盐缓冲盐水中可观察到浑浊的溶液。有机溶剂无法使溶液变澄清。甲醇会产生含有漂浮物的混浊悬浮液,而水会产生均匀的混浊溶液。
该产品可溶于水(5mg / ml)或细胞培养基(1mg / ml),产生浑浊的淡黄色溶液。当涡旋并升温至70-80 oC后,在盐水中可获得更高浓度的溶液(20mg/ml),但仍然是浑浊的溶液。脂多糖是一种在各种溶剂中都形成胶束的分子。在水和磷酸盐缓冲盐水中可观察到浑浊的溶液。有机溶剂无法使溶液变澄清。甲醇会产生含有漂浮物的混浊悬浮液,而水会产生均匀的混浊溶液。
訊號詞
Danger
危險聲明
危險分類
Acute Tox. 2 Oral
儲存類別代碼
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
其他客户在看
Journal of Medical Microbiology, 31, 93-93 (1990)
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To investigate the dysfunction of the immunological barrier of the intestinal mucosa during endotoxemia and to elucidate the potential mechanism of this dysfunction. Male Wistar rats were randomly distributed into two groups: control group and lipopolysaccharide (LPS) group. Endotoxemia was
PloS one, 4(12), e8393-e8393 (2009-12-29)
The pandemic by the novel H1N1 virus has created the need to study any probable effects of that infection in the immune system of the host. Blood was sampled within the first two days of the presentation of signs of
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The immediate early (IE) proteins of human cytomegalovirus (hCMV) have diverse roles in directing viral and host cell transcription. Among these is the ability of IE2 to induce transcription of the IL1B gene that codes for IL-1beta in monocytes. This
Journal of molecular medicine (Berlin, Germany), 89(2), 151-160 (2010-10-26)
The protective effects of high-density lipoprotein (HDL) under lipopolysaccharide (LPS) conditions have been well documented. Here, we investigated whether an effect of HDL on Toll-like receptor 4 (TLR4) expression and signalling may contribute to its endothelial-protective effects and to improved
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