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Key Documents

L5390

Sigma-Aldrich

Lectin from Erythrina cristagalli (coral tree)

lyophilized powder

别名:

Erythrina cristagalli agglutinin, ECA

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About This Item

MDL號碼:
分類程式碼代碼:
12352202
NACRES:
NA.32

形狀

lyophilized powder

品質等級

效力

<0.5 μg per mL agglutination activity

成份

Protein, ≥85% biuret

雜質

salt, free

儲存溫度

−20°C

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生化/生理作用

Lectin or haemagglutinin is a sugar stereospecific binding protein or glycoprotein. It is capable of reversibly agglutinating cells. It is implicated in various cellular processes such as cell agglutination, homing of leukocytes, induction of mitosis in lymphocytes, interferon c (IFN c) and cytotoxicity. It is used as a blood typing reagent in immunohaematology. Lectin is also used as a reagent in testing erythrocyte polyagglutinable states. It is considered as a potential tool for analyzing cell surface architecture and related membrane functions.
ECA is not blood group specific, but has an affinity for D-galactose and D-galactosides. It is reported to be mitogenic for human peripheral blood T lymphocytes.

準備報告

Purified by affinity chromatography.

分析報告

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of trypsinized human blood group O erythrocytes after 1 hr at 25 °C.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Human erythrocyte specific lectin from the seeds of Indian coral tree, Erythrina variegata Linn, var. orientali Linn, Merrill
Datta TK and Basu PS
Journal of Biosciences, 5(1), 25-30 (1983)
Purification, some properties of a D-galactose-binding leaf lectin from Erythrina indica and further characterization of seed lectin
Konozy EHE, et al.
Biochimie, 84(10), 1035-1043 (2002)

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