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Merck

H9664

Sigma-Aldrich

Anti-HMGB1 (HMG1) (N-terminal) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

别名:

Anti-Amphoterin, Anti-High mobility group box 1, Anti-High mobility group protein 1

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.41

生物源

rabbit

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

分子量

antigen 25 kDa

物種活性

human, rat, mouse

加強驗證

independent
Learn more about Antibody Enhanced Validation

技術

immunoprecipitation (IP): 10 μg using HEK-293T cell lysates
indirect immunofluorescence: 1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells.
indirect immunofluorescence: suitable
western blot: 1-2 μg/mL using 3T3 cell lysates

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... HMGB1(3146)
mouse ... Hmgb1(15289)
rat ... Hmgb1(25459)

一般說明

Anti-HMGB1 (HMG1) (N-terminal) is produced in rabbit using as immunogen a synthetic peptide corresponding to human HMGB1 conjugated to KLH. High Mobility Group B (HMGB) protein family includes HMGB1, HMGB2 and HMGB3, that are highly conserved and indistinguishable in their biochemical properties. HMGB1 is a 25 kDa protein of 215 amino acids and consists of two homologous HMG-boxes rich in basic amino acids, and an acidic tail at the carboxy-terminus.
HMGB proteins belong to the High Mobility Group (HMG) family of proteins that contain the HMG-box for binding and changing DNA structures .

免疫原

synthetic peptide corresponding to amino acids 2-17 of human HMGB1, conjugated to KLH. The corresponding sequence is conserved in mouse and rat.

應用

Anti-HMGB1 (HMG1) (N-terminal) antibody is suitable for use in chemiluminescent immunoblot (using mouse heart homogenates) . The antibody can also be used in immunoprecipitation (10 μg using HEK-293T cell lysates), indirect immunofluorescence (1-2 μg/mL using paraformaldehyde-Triton fixed PC12 cultured cells), and western blot (1-2 μg/mL using 3T3 cell lysates).
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

生化/生理作用

High Mobility Group B (HMGB-1 and 2) participate in the regulation of chromatin structure as well as being involved in transcription regulation, DNA repair, recombination, differentiation and extracellular signaling. It shows a defect in transcriptional enhancement of the glucocorticoid receptor, that indicates the important role for HMGB1 in proper transcriptional control by specific transcription factors. Increased expression of HMGB1 is observed in cancer cells.

外觀

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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HMGB1: endogenous danger signaling
Klune J R, et al.
Molecular Medicine, 14(7-8), 476-484 (2008)
David Frescas et al.
Cell cycle (Georgetown, Tex.), 16(16), 1526-1533 (2017-06-27)
Age-related health decline has been attributed to the accumulation of senescent cells recognized in vivo by p16(Ink4a) expression. The pharmacological elimination of p16(Ink4a)-positive cells from the tissues of mice was shown to extend a healthy lifespan. Here, we describe a
J O Thomas
Biochemical Society transactions, 29(Pt 4), 395-401 (2001-08-11)
HMG1 and 2 (high mobility group proteins 1 and 2; renamed HMGB1 and 2) contain two DNA-binding HMG-box domains (A and B) and a long acidic C-terminal domain. They bind DNA without sequence specificity, but have a high affinity for
Michal Stros
Biochimica et biophysica acta, 1799(1-2), 101-113 (2010-02-04)
HMGB proteins are members of the High Mobility Group (HMG) superfamily, possessing a unique DNA-binding domain, the HMG-box, which can bind non-B-type DNA structures (bent, kinked and unwound) with high affinity, and also distort DNA by bending/looping and unwinding. HMGBs
High-mobility group box 1 and cancer
Tang D, et al.
Biochimica et Biophysica Acta - Gene Regulatory Mechanisms, 1799(1-2), 131-140 (2010)

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