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Merck
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文件

H9395

Sigma-Aldrich

α-溶血素 来源于金黄色葡萄球菌

lyophilized powder, Protein ~60 % by Lowry, ≥10,000 units/mg protein

别名:

α-毒素

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About This Item

CAS号:
94716-94-6
MDL號碼:
MFCD00130489
分類程式碼代碼:
12352202
NACRES:
NA.56

生物源

Staphylococcus aureus

品質等級

300

形狀

lyophilized powder

比活性

≥10,000 units/mg protein

包含

sodium citrate buffer as balance

成份

Protein, ~60% Lowry

溶解度

H2O: soluble 0.49-0.51 mg/mL

UniProt登錄號

Q2G1X0

儲存溫度

2-8°C

基因資訊

Staphylococcus aureus ... SAOUHSC_01121(3920722)

一般說明

α-溶血素是一种成孔细胞毒素，它是大多数致病性金黄色葡萄球菌菌株分泌的一种细胞外蛋白。它是以水溶性单体的形式分泌，是一种小型β桶状蛋白。

應用

α-来自金黄色葡萄球菌的溶血素已被用于:
  • 作为电解质溶液的一种成分，用于通过电生理测量测试脂双层中的孔形成
  • 测试其在骨髓基质细胞（BMSCs）中的成骨抑制作用
  • 制备用于 Biacore 和表面等离子体共振的α溶血素分子印迹聚合物 (MIP)

α-溶血素被用于一项研究，用于测试乳制品大肠杆菌的外排泵和溶血素活性。它还用于测试其对苯扎氯铵的适应性以及环丙沙星对生物膜形成的影响。

生化／生理作用

α-溶血素是选择性溶血的，单体形式与膜结合，结合不需要特异性受体。在与生物膜和/或人造膜结合后，它会发生自身低聚化，从而产生代表跨膜孔的环状结构（六聚体），这些孔对离子和小代谢物是通透的。它对兔红细胞有明显的偏好。α-溶血素刺激细胞磷脂酶并诱导 Ca2+流入。它导致内皮屏障的膜破坏和细胞质成分的泄漏和细胞的渗透裂解。α-溶血素与脓毒症的发病机制有关。

包裝

包装尺寸基于蛋白质含量

單位定義

一个溶血单元是在 37°C 下 30 分钟，然后在 4°C 下冷藏 30 分钟后，悬浮在 pH 7.0 磷酸盐缓冲盐水中含1% 牛血清白蛋白的 1% 兔红细胞有50%溶解。

象形圖

Exclamation markHealth hazard
GHS07,GHS08

訊號詞

Warning

危險聲明

H315 - H319 - H371

危險分類

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 2

儲存類別代碼

11 - Combustible Solids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)

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Phosphoinositides (PtdIns) play important roles in exocytosis and are thought to regulate secretory granule docking by co-clustering with the SNARE protein syntaxin to form a docking receptor in the plasma membrane. Here we tested this idea by high-resolution total internal
D Fink et al.
Cellular signalling, 1(4), 387-393 (1989-01-01)
Staphylococcal alpha-toxin at subcytotoxic concentrations stimulated phosphatidylinositol turnover and arachidonic acid release in undifferentiated cultures of pheochromocytoma PC12 cells. Stimulation of phospholipase A2 but not C was dependent on extracellular calcium. Addition of staphylococcal alpha-toxin to PC12 cells caused a
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Frontiers in cellular and infection microbiology, 2, 12-12 (2012-08-25)
One key aspect of the virulence of Staphylococcus aureus lies in its ability to target the host cell membrane with a large number of membrane-damaging toxins and peptides. In this review, we describe the hemolysins, the bi-component leukocidins (which include
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Signalling by heterotrimeric G proteins is often isoform-specific, meaning certain effectors are regulated exclusively by one family of heterotrimers. For example, in excitable cells inwardly rectifying potassium (GIRK) channels are activated by G betagamma dimers derived specifically from G(i/o) heterotrimers.
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