H7165

Anti-HtrA2 (C-terminal) antibody produced in rabbit

~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

• 别名: Anti-High Temperature Requirement protein A2, Anti-HtrA serine peptidase-2, Anti-OMI, Anti-PARK13, Anti-PRSS25, Anti-Protease, Serine, 25
• 分類程式碼代碼: 12352203
• NACRES: NA.41

属性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen ~36 kDa
• 物種活性: rat, human
• 包裝: antibody small pack of 25 μL
• 加強驗證: recombinant expression; Learn more about Antibody Enhanced Validation
• 濃度: ~1.5 mg/mL
• 技術: western blot: 2-4 μg/mL using rat liver microsomal fraction
• UniProt登錄號: O43464
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... HTRA2(27429) ; mouse ... Htra2(64704) ; rat ... Htra2(297376)

说明

一般說明

High temperature requirement protein A2 (HtrA2) or serine peptidase 2 belongs to the HtrA family of serine proteases. It is a mitochondrial serine protease, that comprises of an N-terminal mitochondrial localization sequence (MLS), an inhibitor of apoptosis protein (IAP) binding domain (IBM) and a single C-terminal PDZ domain. It also has a transmembrane and a serine protease domain. HTRA2 gene is mapped to human chromosome 2p13.1.

特異性

Anti-HtrA2 (C-terminal) specifically recognizes human and rat HtrA2.

應用

Anti-HtrA2 (C-terminal) antibody produced in rabbit may been used in immunoblotting.

生化／生理作用

High temperature requirement protein A2 (HtrA2) exhibits endoproteolytic activity by cleaving misfolded proteins and other cellular proteins. It is essential for mitochondrial homeostasis and regulates apoptosis through multiple pathways, including caspase-dependent and caspase-independent cell death. Mature HtrA2, via its IAP binding domain favors the neutralizing inhibitor of apoptosis proteins (IAPs) by promoting the cytochrome c-dependent caspase activation. Mutations that inactivate HtrA2 gene are associated with neurodegenerative diseases such as Parkinson′s disease and Alzheimer′s disease. Mutation in the HtrA2 gene is also implicated in the early-onset mitochondrial syndromes including the 3-methylglutaconic aciduria. HtrA2 variants are responsible infantile neurodegeneration, mitochondrial dysfunction and promote apoptosis.

外觀

Solution in 0.01 M phos­phate buffered saline, pH 7.4, containing 15 mM sodium azide.

儲存和穩定性

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全信息

• 儲存類別代碼: 10 - Combustible liquids
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)