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形狀
solution
用途
sufficient for 25 blots
特點
wetted part: no
製造商／商標名
Cytiva RPN810
包裝
pkg of 25 loadings
運輸包裝
dry ice
儲存溫度
−20°C
一般說明
ECL™ DualVue Western Blotting Markers contain both colored proteins and tagged proteins that introduce convenience and reliability and facilitate accurate size determination of proteins on Western blots.
應用
Amersham™ ECL™ DualVue Western Blotting Markers perform two key functions. First, the prestained marker Proteins confirm Protein transfer in addition to clearly defining blot orientation. Second, the recombinant tagged Proteins are detected on film or by CCD imaging in conjunction with the target Protein(s) of interest, enabling highly accurate molecular mass determination of the target protein(s). The tagged markers are detected easily and specifically by means of a specific conjugate that eliminates any cross contamination between target Protein(s) and markers.
Amersham™ ECL™ DualVue Western Blotting Markers contain a mixture of three prestained colored markers of defined molecular weight (from 15 000 to 100 000) and seven tagged recombinant Proteins (molecular weights from 15 000 to 150 000). Each pack contains ECL™ DualVue markers (125 μL) and S-Protein-HRP conjugate (50 μL), sufficient for 25 standard gel loadings.
Amersham™ ECL™ DualVue Western Blotting Markers contain a mixture of three prestained colored markers of defined molecular weight (from 15 000 to 100 000) and seven tagged recombinant Proteins (molecular weights from 15 000 to 150 000). Each pack contains ECL™ DualVue markers (125 μL) and S-Protein-HRP conjugate (50 μL), sufficient for 25 standard gel loadings.
特點和優勢
- Detect Proteins on gel and membrane as well as on film.
- Prestained markers indicate efficiency of Protein transfer to membrane and blot orientation.
- Tagged recombinant Protein markers allow accurate calculation of molecular weight of target Protein(s).
- Product is compatible with any HRP substrate as well as both PVDF and nitrocellulose membrane types.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Amersham is a trademark of Cytiva
ECL is a trademark of Cytiva
儲存類別代碼
12 - Non Combustible Liquids
John Kerwin et al.
eLife, 9 (2020-04-22)
As part of the Reproducibility Project: Cancer Biology we published a Registered Report (Khan et al., 2015), that described how we intended to replicate selected experiments from the paper "A coding-independent function of gene and pseudogene mRNAs regulates tumour biology"
商品
Troubleshooting of Detection Methods of GST-tagged Proteins
实验方案
This page describes chemiluminescence detection of GST-tagged proteins with Amersham ECL, Amersham ECL Prime, and Amersham ECL Select from GE Healthcare.
This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using GE Healthcare products.
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