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Merck
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主要文件

M3546

Sigma-Aldrich

超低范围分子量标记物(M.W. 1,060-26,600)

别名:

protein markers, protein molecular weight markers, protein standards

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About This Item

分類程式碼代碼:
41105337
NACRES:
NA.25

用途

 vial sufficient for 400 applications on large gels (16 × 18 cm)
 vial sufficient for 800 mini-gels (10 × 10 cm)

包裝

vial of 200 μL

儲存溫度

−20°C

應用

超低分子量标记物 (M.W.1,060-26,600) 已作为蛋白质标记物用于十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 和免疫印迹。

生化/生理作用

超低量程标记物设计用于 Tris-Tricine SDS-PAGE 方法 当用亮蓝 G(非甲醇基)染色时。建议使用超低标记物固定戊二醛,顺序使两种最小的蛋白质固定在凝胶中。超低量程标记物以液体形式提供,上样前必须用提供的样品缓冲液进行稀释 (1:20)。

外觀

0.1 MTris-HCl 溶液,pH 值约为 8.5,与 4 mM EDTA,3 mM 叠氮化钠和 40% 甘油

訊號詞

Danger

危險分類

Acute Tox. 4 Dermal - Aquatic Chronic 3 - Eye Dam. 1 - Repr. 2 - Skin Sens. 1

儲存類別代碼

10 - Combustible liquids

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized C-terminal part of divercin V41 (DvnCt) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and reactivity of the DvnCt-KLH-generated antibodies were evaluated by enzyme-linked
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Toxins, 12(6) (2020-06-19)
The biological activity of Rhinella icterica parotoid secretion (RIPS) and some of its chromatographic fractions (RI18, RI19, RI23, and RI24) was evaluated in the current study. Mass spectrometry of these fractions indicated the presence of sarmentogenin, argentinogenin, (5β,12β)-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide, marinobufagin, bufogenin
H Schägger et al.
Analytical biochemistry, 166(2), 368-379 (1987-11-01)
A discontinuous sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) system for the separation of proteins in the range from 1 to 100 kDa is described. Tricine, used as the trailing ion, allows a resolution of small proteins at lower acrylamide concentrations

实验方案

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)

Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)

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