跳转至内容
Merck
所有图片(1)

主要文件

GE28-9523-60

GST GraviTrap

Cytiva 28-9523-60

登录查看公司和协议定价


About This Item

分類程式碼代碼:
41106501
NACRES:
NA.56

ligand

glutathione

包裝

pkg of 10 ea

製造商/商標名

Cytiva 28-9523-60

床體積

2 mL

柱內徑

15 mm

基質

4% agarose

粒徑

45-165 μm

平均直徑

90 μm

cleaning

4-13

工作範圍

4-13

容量

≥20 mg binding capacity(recombinant glutathione S-transferase (rGST))
>25 mg binding capacity (horse liver GST/ml medium)

一般說明

Binding of GST to glutathione is flow-rate dependent and lower flow rates often increase the binding capacity. This is important to consider during sample loading and elution.
GST GraviTrap contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion protein.

應用

GST GraviTrap contains 10 disposable columns prepacked with 2 mL of Glutathione Sepharose 4B, sufficient for purification of up to 20 mg of fusion Protein. GST-tagged Proteins are recovered under mild elution conditions (10 mM glutathione), which preserves functionality of the Proteins. The binding capacity of prepacked GST GraviTrap columns, which can be greater than 10 mg Protein/mL medium, varies with the nature of the tagged Protein and the exact binding conditions used. For increased convenience, GST Buffer Kit contains stock solutions of the buffers needed for purification of GST-tagged Proteins.

特點和優勢

  • Convenient, prepacked gravity-fLow columns.
  • Allows direct purification of both clarified and unclarified cell lysates.
  • Yields up to 20 mg of pure Proteins per column.
  • Yields up to 20 mg of pure Proteins per column

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

GraviTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助,请联系 客户支持

已有该产品?

在文件库中查找您最近购买产品的文档。

访问文档库

商品

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page shows troubleshooting methods for purification of GST-tagged proteins using GE Healthcare products.

This page describes characteristics of Glutathione Sepharose and HiTrap Benzamidine FF (high sub) media and columns from GE Healthcare.

This page describes troubleshooting strategies in purification of GST-tagged proteins using GE Healthcare products.

实验方案

Characteristics of Glutathione Sepharose® Products

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门