跳转至内容
Merck
所有图片(1)

Key Documents

GE28-9440-08

Protein G Mag Sepharose™

Cytiva 28-9440-08, pack of 1 × 500 μL

登录查看公司和协议定价
所有图片(1)

About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

protein G

包裝

pack of 1 × 500 μL

製造商／商標名

Cytiva 28-9440-08

儲存條件

(20% Ethanol, 20% medium slurry)

參數

Room temperature temp. range

基質

paramagnetic, spherical, highly cross-linked agarose particles

容量

13- 22 mg binding capacity(human IgG/ml medium)

儲存溫度

2-8°C

一般說明

Protein G Mag Sepharose™ are magnetic beads designed to simplify enrichment of target proteins by immunoprecipitation applications. Based on Sepharose™ with protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies.

應用

Protein G Mag Sepharose™ are magnetic beads designed to simplify enrichment of target Proteins by immunoprecipitation or pull-down applications. The magnetic beads are based on Sepharose™ with Protein G as ligand. Protein G has high affinity for the Fc region of IgG antibodies from a broad variety of species.

There are two protocols available for Protein enrichment: the cross-link protocol and the classic protocol. The protocols are simple with elution conditions optimized for both electrophoresis and MS analysis.

Protein G Mag Sepharose™ combines well-proven enrichment methods with the magnetic beads platform, which has excellent properties for small-scale sample preparation. Together with MagRack 6, a separation tool for six microcentrifuge tubes, up to six samples can be processed in parallel.

特點和優勢

  • Visible and dense Sepharose™ based magnetic beads with Protein A ligand, easy to spot and collect bound target Protein
  • Non-adherent beads eliminate smearing effects and aggregate formation. Can be used without detergents
  • Simple capture of target Protein in small or large sample volumes (Low microliter to high milliliter scale)
  • Optimized capacity for enrichment/immunoprecipitation of target Proteins - only Low amounts of antibody needed
  • Flexible protocols with elution conditions optimized for both electrophoresis and MS analysis

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

商品

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

实验方案

Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra

This page shows how to separate IgG antibodies by affinity chromatography using Protein A Mag Sepharose Xtra or Protein G Mag Sepharose Xtra from GE Healthcare.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门