推荐产品
ligand
dextrin
包裝
pack of 100 mL
製造商/商標名
Cytiva 28-9355-98
儲存條件
(20% Ehtanol)
基質
highly cross-linked 6% agarose
樣品平均尺寸
34 μm
cleaning in place
2-13
工作範圍
>7
容量
~7 mg binding capacity (MBP- ΔSal/ml medium Approx. 16 mg MBP- ßGal/ml medium)
儲存溫度
2-8°C
一般說明
Binding capacity is protein dependent. Dynamic binding capacity was tested with MBP-?Sal (MBP2*-paramyosin-?Sal, Mr approx. 70 000, multimer in solution), and MBP-ßGal (MBP2*-beta-galactosidase, Mr approx. 158 000, multimer in solution).
Dextrin Sepharose™ High Perfromance is an affinity media for purification of proteins tagged with Maltose Binding protein (MBP).
應用
Dextrin Sepharose™ High Performance and provide convenient affinity purification of recombinant Proteins tagged with maltose binding Protein (MBP). This robust and stable Sepharose™ High Performance based medium enables elution of target Protein in narrow peaks, which minimizes the need for further concentration steps.
特點和優勢
- Dextrin Sepharose™ High Performance is a robust, high resolution medium based on the 34 μm Sepharose™ High Performance matrix
- The small, evenly sized beads ensure that MBP-tagged Proteins elute in narrow peaks, thus minimizing the need for further concentration steps
- Physiological conditions and mild elution preserve target Protein activity
- Dextrin Sepharose™ High Performance tolerates all commonly used aqueous buffers and is easily regenerated using 0.5 M NaOH allowing the same column to be used for repeated purifications
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
Sepharose is a trademark of Cytiva
訊號詞
Warning
危險聲明
儲存類別代碼
3 - Flammable liquids
实验方案
This page describes efficient column packing and preparation for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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