推荐产品
製造商/商標名
Cytiva 27-5325-01
包裝
pkg of 50 columns
粒徑
20-80 μm (dry)
工作範圍
2-13
一般說明
MicroSpin™ G-25 columns are designed for the rapid purification of DNA for use in a wide range of applications, including desalting, buffer exchange, and removal of unincorporated nucleotides from end-labelled oligonucleotides.
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
MicroSpin™ G-25 columns can be used for any DNA greater than 10 bases in length and are therefore an excellent choice for the purification of oligonucleotides or very small DNA fragments following synthesis or a labelling reaction. They will not remove or denature enzyme.
特點和優勢
- For rapid buffer exchange/desalting of PCR products and other DNAs in a volume of 10 to 100 μl using spin-column chromatography.
- Excellent for rapid purification of newly synthesized oligonucleotides >10-mers in 100 to 150 μl of deprotection solution using spin-column chromatography.
- Convenient: Prepacked with Sephadex® G-25 DNA Grade and pre-equilibrated in distilled water containing 0.05% Kathon CG/ICP Biocide.
- Ready to use: Requires less than 4 min from sample application to collection of purified product.
- Tested in nickase, single and double-stranded exonuclease and RNase assays.
- Can also be used for desalting/buffer exchange of DNA and removal of unincorporated radionucleotides from end-labeled oligonucleotides (at least 10 bases in length) in a volume of 10 to 100 μl.
儲存和穩定性
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 20 to 25 °C. (Double distilled water containing 0.05% Kathon CG/ICP Biocide as preservative)
分析報告
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
法律資訊
MicroSpin is a trademark of Cytiva
Sephadex is a registered trademark of Cytiva
儲存類別代碼
12 - Non Combustible Liquids
商品
This page shows how to solve practical problems that may occur when running an affinity chromatography column.
实验方案
This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.
How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.
This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.
This page shows how to prepare samples for purification with affinity chromatography.
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