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GE17-1408-01

HiTrap® Desalting Columns

Cytiva 17-1408-01, pack of 5 × 5 mL

别名:

Purification Columns

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About This Item

分類程式碼代碼:
23151816
NACRES:
NA.56

包裝

pack of 5 × 5 mL

製造商/商標名

Cytiva 17-1408-01

儲存條件

dry media

參數

42 psi

床尺寸

16 mm × 25 mm

床體積

5 mL

柱內徑

16 mm

基質

cross-linked dextran

粒徑

>20 μm (dry)

cleaning

2-13

工作範圍

2-13

適合性

suitable for bioprocess medium

一般說明

HiTrap® Desalting is a ready-to-use 5 mL column, prepacked with Sephadex® G-25 Superfine, for fast and convenient desalting and buffer exchange.

HiTrap® Desalting is a prepacked, ready to use column for group separation between high and low molecular weight substances, i.e., buffer exchange prior to or after different chromatographic steps, removal of low molecular weight contaminants or removal of reagents to terminate a reaction.

應用

Desalting and buffer exchange with Sephadex® G-25.

特點和優勢

  • Handles sample volumes of 1.5 mL, or larger volumes by connecting up to five columns in series.
  • Replaces dialysis as the method of choice for desalting.
  • Desalting requires less than one minute due to the high rigidity of Sephadex® G-25 and the optimized packing technology.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (4 to 8, Used Media 20% Ehtanol or 0.01 M NaOH)

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

其他說明

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

法律資訊

HiTrap is a registered trademark of Cytiva
Sephadex is a registered trademark of Cytiva

象形圖

Flame

訊號詞

Warning

危險聲明

儲存類別代碼

3 - Flammable liquids


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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商品

This page describes general considerations for purification of GST-tagged proteins using GE Healthcare products.

This page shows how to perform a purification and on-column refolding of an insoluble his-tagged protein from an E. coli culture with HisTrap FF columns and ÄKTAprime from GE Healthcare.

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

This page shows how to condition membrane proteins for further analysis with products from GE Healthcare.

How to perform buffer exchange and desalting with Sephadex G-25, HiTrap Desalting columns, or ÄKTAprime plus.

This page clarifies sample preparation, buffer exchange and desalting, removal of lipoproteins, phenol red, and low molecular weight contaminants in Ion exchange chromatography.

This page shows how to prepare samples for purification with affinity chromatography.

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