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Key Documents

GE17-0618-02

Protein G Sepharose™ 4 Fast Flow

Cytiva 17-0618-02, pack of 25 mL

别名:

Sepharose 4 Fast Flow

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About This Item

分類程式碼代碼:
41106500
NACRES:
NA.56

ligand

recombinant protein G lacking albumin-binding region

包裝

pack of 25 mL

製造商／商標名

Cytiva 17-0618-02

儲存條件

(20% Ehtanol)

基質

4% cross-linked agarose

平均直徑

90 μm (d50v)

cleaning in place

2-10

工作範圍

3-9

適合性

suitable for bioprocess medium

儲存溫度

2-8°C

相关类别

一般說明

Protein G Sepharose™ 4 Fast Flow is recombinant protein G coupled to Sepharose™ 4 Fast Flow.

Protein G Sepharose™ 4 Fast Flow has recombinant protein G immobilized by the cyanogen bromide (CNBr) method to Sepharose™ 4 Fast Flow. Protein G exhibit binding specificities that complement Protein A media and binds to the Fc region of IgG from a variety of mammalian species. Protein G Sepharose™ 4 Fast Flow may be used to isolate and purify classes, subclasses and fragments of immunoglobulins from any biological fluid or cell culture medium.

As member of the BioProcess media range, Protein G Sepharose™ 4 Fast Flow meets industrial demands with security of supply and comprehensive technical and regulatory support.

特點和優勢

  • Binding specificities that complement Protein A media.
  • Binds a broad range of IgG species and subclasses.
  • Multi-point attachment minimizes ligand leakage.
  • Used in a range of research applications.

儲存和穩定性

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

分析報告

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

法律資訊

Sepharose is a trademark of Cytiva

象形圖

Flame
GHS02

訊號詞

Warning

危險聲明

H226

儲存類別代碼

3 - Flammable liquids

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商品

Immunoprecipitation Techniques

This page describes immunoprecipitation (immunoaffinity or pull-down techniques).

Column Packing and Preparation for Affinity Chromatography of Antibodies

This page describes efficient column packing and preparation for affinity chromatography of antibodies.

Desalting and Buffer Exchange for Affinity Chromatography of Antibodies

This page shows how to perform sample desalting and buffer exchange for affinity chromatography of antibodies.

Affinity Chromatography Troubleshooting

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

实验方案

Pull-down assays

This page provides information about different pull-down assays for the further isolation of multiprotein complexes to identify their components with products from GE Healthcare.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

Sample Preparation for Affinity Chromatography

This page shows how to prepare samples for purification with affinity chromatography.

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