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Merck

G9293

Sigma-Aldrich

Anti-GAL4, Activation domain antibody produced in rabbit

~1.0 mg/mL, affinity isolated antibody, buffered aqueous solution

别名:

Anti-Galactose 4

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.56

生物源

rabbit

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

濃度

~1.0 mg/mL

技術

western blot: 0.5 μg/mL using GAL4-AD fusion protein in Saccharomyces cerevisiae extract

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

一般說明

GAL4 protein is an 881 amino acid transcription factor containing an independent N-terminal DNA binding (147 amino acids) and C-terminal activator domains. It is a regulatory gene that belongs to GAL gene family. It is a component of yeast two hybrid system, where GAL 4 DNA-BD is fused to protein X (bait) and GAL4 activation domain is fused to protein Y (prey).
Useful in the study of protein:protein interaction studies. GAL4 DNA activation domain fusion proteins.

免疫原

Synthetic peptide corresponding to amino acids of Saccharomyces cerevisiae GAL4 protein.

應用

Anti-GAL4, Activation domain antibody produced in rabbit has been used in western blotting.

生化/生理作用

GAL4 induces the genes that are responsible for regulation of galactose metabolism in saccharomyces cerevisiae. GAL4 protein is widely used for studying transcriptional activation in eukaryotes. The yeast two hybrid system is widely used to study to discover protein− protein interactions (PPIs) and protein−DNA interactions.

外觀

The product is provided as a solution of affinity isolated antibody in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide as a preservative.

儲存和穩定性

For continuous use, store at 2-8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免責聲明

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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H Efsun Arda et al.
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Genetics, 183(1), 13-22 (2009-07-15)
Reversible and easy to use, temperature-sensitive (TS) mutations are powerful tools for studying gene function. However, TS alleles are rare and difficult to generate and identify, and this has limited their use in most multicellular organisms. We have generated and
Alessandro Berto et al.
EMBO reports, 19(5) (2018-04-11)
Cenp-F is a multifaceted protein implicated in cancer and developmental pathologies. The Cenp-F C-terminal region contains overlapping binding sites for numerous proteins that contribute to its functions throughout the cell cycle. Here, we focus on the nuclear pore protein Nup133

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