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Merck

G6142

Sigma-Aldrich

甘油激酶 来源于纤维单胞菌

lyophilized powder, 25-75 units/mg protein

别名:

ATP:甘油3-磷酸转移酶, 甘油激酶

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About This Item

CAS号:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

形狀

lyophilized powder

比活性

25-75 units/mg protein

分子量

~128 kDa (by gel filtration)

成份

Protein, ≥60% biuret

儲存溫度

−20°C

一般說明

Research area: Cell Signaling

Glycerol kinase (GK) is part of the FGGY carbohydrate kinase family.

應用

Glycerokinase from Cellulomonas sp. has been used:
  • for determining the kinetic characteristics of human and trypanosomatid phosphofructokinases using an enzyme-linked kinetic assay.
  • to study the effect of sugar in fluorescence emission.
  • in 2-Arachidonoylglycerol-based fluorescence assay for DH-463, a fluorescent activity-based probe for monoacylglycerol lipase.

生化/生理作用

甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸,并且是甘油利用中的限速酶。 它也受到果糖-1,6-二磷酸的反馈调节。

物理性質

等电点:4.2
米氏常数:4.4 x 10-5M(甘油),4.3 x 10-4M(ATP)
抑制剂:对对氯汞苯甲酸,重金属离子(Pb++,Fe++,Hg++,Ag+
最佳pH:9.8(G-3-PDH系统),7.8(G-3-P氧化酶系统)最佳温度:500C
pH稳定性:pH 5.5 x 10.0 (25oC, 20hr)
热稳定性:低于40oC(pH 7.5,15min)
底物特异性:这种酶催化ATP的磷酰基部分末端的立体特异性转移
到甘油的一个伯羟基,
形成sn-甘油-3-P。酶具有最高的
甘油特异性,也能磷酸化二羟基丙酮
和甘油醛(表1,2)。Mg++ 基本上是反应必需的。
.

單位定義

一个单位为在pH 9.8和25℃下,在PK/LDH的偶联体系内每分钟将1.0 μmole甘油和ATP转化为L-α甘油磷酸盐和ADP。

外觀

冻干粉含磷酸盐缓冲盐和葡萄糖酸钠

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Purification and properties of glycerol kinase from Escherichia coli.
S I Hayashi et al.
The Journal of biological chemistry, 242(5), 1030-1035 (1967-03-10)
Zhongya Qin et al.
Biomedical optics express, 9(7), 3373-3390 (2018-07-10)
The femtosecond laser ablation in biological tissue produces highly fluorescent compounds that are of great significance for intrinsically labelling ablated tissue in vivo and achieving imaging-guided laser microsurgery. In this study, we analyzed the molecular structures of femtosecond laser-ablated tissues
N Zwaig et al.
Journal of bacteriology, 102(3), 753-759 (1970-06-01)
The activity of glycerol kinase is rate-limiting in the metabolism of glycerol by cells of Escherichia coli. A mutant strain producing a glycerol kinase resistant to inhibition by fructose-1,6-diphosphate grows faster than its wild-type parent on glycerol as the sole
Ioanna Gouni-Berthold et al.
The British journal of nutrition, 107(11), 1694-1706 (2011-10-15)
Animal and human studies suggest that a malleable protein matrix (MPM) from whey decreases plasma lipid concentrations and may positively influence other components of the metabolic syndrome such as glucose metabolism and blood pressure (BP). The primary objective of this
Daria V Cherkashina et al.
Rejuvenation research, 14(6), 661-667 (2011-10-20)
Abstract Effects of stem and progenitor cells or their compounds on recipient cells are investigated intensively today. In spite of this, their ability to interact with native cells and the final targets affected by them, particularly biochemical parameters that characterize

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