推荐产品
生物源
rabbit
共軛
unconjugated
抗體表格
affinity isolated antibody
抗體產品種類
primary antibodies
無性繁殖
polyclonal
形狀
buffered aqueous solution
物種活性
human
濃度
~1.0 mg/mL
技術
indirect immunofluorescence: 2.5-5.0 μg/mL using human epithelial HEp-2 cells
UniProt登錄號
運輸包裝
dry ice
儲存溫度
−20°C
目標翻譯後修改
unmodified
基因資訊
mouse ... Tnrc6a(233833)
rat ... Tnrc6a(308971)
一般說明
GW182 is a phosphorylated cytoplasmic autoantigen. It contains multiple glycine-tryptophan (GW) repeats along with a single RNA recognition motif. GW182 is a protein of 182 kDa, found in mammalian cell types.
應用
Anti-GW182 antibody produced in rabbit has been used as a primary antibody for immunostaining of HeLa cells and human hepatoma cells. It has also been used for indirect immunofluorescence at a working concentration of 2.5-5.0μg/mL using human epithelial HEp-2 cells.
生化／生理作用
Glycine-tryptophan (GW) bodies or P-bodies are cytoplasmic bodies that are involved in mRNA degradation, storage and translational repression. These bodies contain Argonaute proteins and m-RNAs that associate with GW182. GW182 is involved in post-transcriptional gene silencing/RNA interference (RNAi) via the microRNA pathway. Inhibition of gene expression leads to destruction of GWBs and disruption of RNAi and microRNA-induced gene silencing as it forms an integral component of GWBs.
外觀
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免責聲明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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儲存類別代碼
10 - Combustible liquids
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
GW182 is critical for the stability of GW bodies expressed during the cell cycle and cell proliferation
Yang Z, et al.
Journal of Cell Science, 117(23), 5567-5578 (2004)
A phosphorylated cytoplasmic autoantigen, GW182, associates with a unique population of human mRNAs within novel cytoplasmic speckles
Eystathioy T, et al.
Molecular Biology of the Cell, 13(4), 1338-1351 (2002)
Andrew Jakymiw et al.
Journal of cell science, 120(Pt 8), 1317-1323 (2007-04-03)
GW bodies, also known as mammalian P-bodies, are cytoplasmic foci involved in the post-transcriptional regulation of eukaryotic gene expression. Recently, GW bodies have been linked to RNA interference and demonstrated to be important for short-interfering-RNA- and microRNA-mediated mRNA decay and
Andrew Jakymiw et al.
Nature cell biology, 7(12), 1267-1274 (2005-11-15)
The GW182 RNA-binding protein was initially shown to associate with a specific subset of mRNAs and to reside within discrete cytoplasmic foci named GW bodies (GWBs). GWBs are enriched in proteins that are involved in mRNA degradation. Recent reports have
Zheng Yang et al.
Journal of cell science, 117(Pt 23), 5567-5578 (2004-10-21)
A novel cytoplasmic compartment referred to as GW bodies was initially identified using human autoantibodies to a 182 kDa protein named GW182. GW bodies are small, generally spherical, cytoplasmic domains that vary in number and size in several mammalian cell
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