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Merck

G2150

Sigma-Aldrich

L -谷氨酰胺 溶液

200 mM, Hybri-Max, sterile-filtered, suitable for hybridoma

别名:

L-Glutamine for cell culture

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About This Item

CAS号:
MDL號碼:
分類程式碼代碼:
12352209
eCl@ss:
32160406
PubChem物質ID:
NACRES:
NA.75

等級

Hybri-Max

品質等級

無菌

sterile-filtered

化驗

≥99% (TLC)

形狀

solution

技術

cell culture | hybridoma: suitable

雜質

endotoxin, tested

顏色

clear colorless

運輸包裝

dry ice

儲存溫度

−20°C

SMILES 字串

N[C@@H](CCC(N)=O)C(O)=O

InChI

1S/C5H10N2O3/c6-3(5(9)10)1-2-4(7)8/h3H,1-2,6H2,(H2,7,8)(H,9,10)/t3-/m0/s1

InChI 密鑰

ZDXPYRJPNDTMRX-VKHMYHEASA-N

基因資訊

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一般說明

L-谷氨酰胺是一种必需氨基酸,作为细胞培养过程中主要的能量来源而成为培养基的重要组分。L-谷氨酰胺在干粉和冷冻溶液中非常稳定。然而,在液体培养基或储备溶液中,L-谷氨酰胺的降解速度相对更快。为获得最佳细胞性能,通常需要在使用培养基前补充L-谷氨酰胺。

應用

L-谷氨酰胺溶液已用作罗斯威尔公园纪念研究所(RPMI)-1640培养基的补充剂,以培养人白血病单核细胞系用于细胞内分析和脾细胞用于CD4+ T细胞的体外激活。它还用作杂交瘤培养基的补充剂,用于抗原结合和杂交瘤克隆的初级筛选。

準備報告

使用细胞培养级水进行制备。

法律資訊

Hybri-Max is a trademark of Sigma-Aldrich Co. LLC

儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

nwg

閃點(°F)

Not applicable

閃點(°C)

Not applicable


历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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Production of human antibodies from transgenic mice.
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Methods in molecular biology (Clifton, N.J.), 248, 191-200 (2004-02-19)
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Screening of a GSK-proprietary library against intracellular Mycobacterium tuberculosis identified 1, a thioalkylbenzoxazole hit. Biological profiling and mutant analysis revealed that this compound is a prodrug that is bioactivated by the mycobacterial enzyme MymA. A hit-expansion program including design, synthesis
Glutamine and antioxidants in critically ill patients.
Daren Heyland et al.
The New England journal of medicine, 369(5), 484-485 (2013-08-02)
A U Lekmanov et al.
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Comparing analysis of randomized study was conducted. 40 Pediatric patients (2-15 years old) with heavy thermic burns and concomitant injuries were recruited into the study to assess the glutamine solution intravenous use efficiency. Parenteral feeding and feeding with glutamine solution
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Cancer cells are markedly different from normal cells with regards to how their metabolic pathways are used to fuel cellular growth and survival. Two basic metabolites that exemplify these differences through increased uptake and altered metabolic usage are glucose and

商品

L-谷氨酰胺在杂交瘤细胞和哺乳动物细胞培养中的重要性和应用

Importance and uses of glutamine in hybridoma and mammalian cell culture

Chromatograms

application for HPLC

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