G1270

L-谷氨酰胺合成酶 来源于大肠杆菌

lyophilized powder, 400-2,000 units/mg protein

• 别名: L-Glutamate:ammonia ligase (ADP-forming)
• CAS号: 9023-70-5
• EC 号: 6.3.1.2 (BRENDA, IUBMB)
• MDL號碼: MFCD00131463
• 分類程式碼代碼: 12352204
• NACRES: NA.26

属性

• 形狀: lyophilized powder
• 品質等級: 200
• 比活性: 400-2,000 units/mg protein
• 純化經由: affinity chromatography
• 包含: dithioerythritol as preservative
• 成份: Protein, ~5% Lowry
• 溶解度: H₂O: soluble 0.95-1.05 mg/mL, clear to hazy
• UniProt登錄號: P0A9C5
• 異物活動: ATPase <0.2%
• 儲存溫度: −20°C
• 基因資訊: Escherichia coli K12 ... glnA(948370)

说明

一般說明

L-Glutamine Synthetase from bacteria shows dodecameric structure comprising of 12 active sites. Each active site termed bifunnel, has an ATP and glutamate binding sites. The dodecamer is stabilized by two hexameric rings.

應用

L-Glutamine Synthetase from Escherichia coli has been used in the synthesis of methylglutamine from methylammonium in E coli and in the glutamine synthetase protection activity of human thioredoxin peroxidase enzyme, AOE372.

L-Glutamine synthetase may be used for the purification of proteases from Escherichia coli.

生化／生理作用

L-glutamine synthetase catalyzes the condensation of L-glutamate and ammonia to L-glutamine. It is a degradative enzyme for glutamic acid.

Nitrogen starvation dictates the expression of the glutamine synthetase (GS) gene in E. coli. GS plays a key role in ammonia assimilation in bacteria. Adenylylation of GS is catalyzed by adenylyltransferase. Adenylylation of GS modulates its catalytic functionality resulting in glutamine limitation in E coli.

谷氨酸降解酶

單位定義

One unit will convert 1.0 μmole of L-glutamate to L-glutamine in 15 min at pH 7.1 at 37 °C.

外觀

Contains potassium phosphate, sodium citrate and magnesium acetate buffer salts

安全信息

• 象形圖: GHS08
• 訊號詞: Danger
• 危險聲明: H334
• 防範說明: P261 - P284 - P501
• 危險分類: Resp. Sens. 1
• 儲存類別代碼: 11 - Combustible Solids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, type N95 (US)