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形狀
lyophilized powder
品質等級
比活性
≥150 units/mg protein (biuret)
成份
Protein, 5.0-20.0%
UniProt登錄號
異物活動
adenosine triphosphatase and NADH oxidase ≤0.01%
儲存溫度
−20°C
基因資訊
Escherichia coli K12 ... ackA(946775)
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一般說明
乙酸激酶属于乙酸盐和糖激酶/Hsc70/肌动蛋白(ASKHA)结构超家族。它以二聚体形式存在,并在N和C端结构域之间的缝隙中存在有核苷酸结合位点。
應用
乙酸激酶可用于将乙酸磷酸化为乙酰磷酸。 来自大肠杆菌的乙酸激酶已被用作ATP再生系统的一部分,用于研究激动剂刺激的转磷脂酰化反应的动力学。
在木糖葡糖杆菌的发酵过程以及动力学建模和稳态研究中,来自大肠杆菌的乙酸激酶已被用于评估乙酸激酶的活性。
生化/生理作用
乙酸激酶在糖酵解中起重要作用。乙酸激酶会在ATP和二价阳离子的存在下将乙酸盐磷酸化,并最终导致乙酰辅酶A的生成。乙酸激酶也会参与到丙酸酯、丙酮酸和牛磺酸的代谢。
参与丙酸酯、丙酮酸酯和牛磺酸的代谢。
單位定義
在pH 7.6、25℃条件下,一单位可在每分钟内将1.0 μmole的乙酸磷酸化成乙酰磷酸。
外觀
冻干粉,含海藻糖,以及少量磷酸钾、氯化镁和二硫苏糖醇
儲存類別代碼
11 - Combustible Solids
水污染物質分類(WGK)
WGK 3
閃點(°F)
Not applicable
閃點(°C)
Not applicable
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Influence of residual ethanol concentration on the growth of Gluconacetobacter xylinus I 2281
Applied Microbiology and Biotechnology, 62(2-3), 168-173 (2003)
Urkinase: structure of acetate kinase, a member of the ASKHA superfamily of phosphotransferases
Journal of Bacteriology, 183(2), 680-686 (2001)
The Journal of biological chemistry, 266(26), 17236-17242 (1991-09-15)
Receptor-linked activation of phospholipase D has been demonstrated recently in a variety of intact cell types including granulocytes, but little is known about the enzyme, its cofactor requirements, and regulation. Using [3H]alkyllysophosphatidylcholine to prelable an endogenous phosphatidylcholine substrate pool in
Journal of bacteriology, 175(22), 7348-7355 (1993-11-01)
The Bacillus subtilis gene encoding acetate kinase was identified on the basis of sequence similarity to the Escherichia coli ackA gene and to a second E. coli gene closely related to ackA. Insertional inactivation of this region of the B.
Frontiers in microbiology, 11, 156-156 (2020-03-21)
The cell surface is the primary recognition site between the bacterium and the host. An operon of three genes, LSEI_0219 (cwaR), LSEI_0220 (cwaS), and LSEI_0221 (ldcA), has been previously identified as required for the establishment of Lactobacillus paracasei in the
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