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Merck
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Key Documents

F4135

Sigma-Aldrich

胎牛血清

USA origin, Heat Inactivated, sterile-filtered, suitable for cell culture, suitable for insect cell culture, suitable for hybridoma

别名:

HI FBS, HI FCS, HI 血清, FBS, FCS, 血清

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About This Item

MDL號碼:
MFCD00132239
分類程式碼代碼:
12352207
eCl@ss:
32160409
NACRES:
NA.71

生物源

bovine fetus

品質等級

500

無菌

sterile-filtered

產品線

FBS Classic

成份

Bovine IgG, ≤1 mg/mL
Hemoglobin, ≤20 mg/dL

來源

USA origin

技術

cell culture | hybridoma: suitable
cell culture | insect: suitable
cell culture | mammalian: suitable

雜質

≤10 EU/mL endotoxin

運輸包裝

dry ice

儲存溫度

−20°C

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一般說明

胎牛血清（FBS）一直是一种常用的培养基补充剂，因为它在细胞培养中提供了广泛的功能。FBS提供营养物、生长和附着因子，并有助于增殖。它还保护细胞免于氧化损伤和细胞凋亡。FBS阻断活性氧的生成，否则在无血清培养基（SFM）系统中不会消除这种活性氧。

應用

来自美国的FBS被广泛用于细胞培养应用中。FBS可为培养的细胞提供了多种未确定的生长促进和存活增强因子。
胎牛血清已用于：
  • 作为Dulbecco′s Modified Eagles Media (DME, DMEM)、 Eagle′s Minimum Essential Medium (EMEM)、 Roswell Park Memorial Institute 培养基 (RPMI-1640)、 α-改良 eagle培养基(αMEM) (MEM)和 Leibovitz L-15培养基的成分
  • 作为处理脑干切片的封闭介质的一种成分
  • 用于改善真皮外植体的附着

準備報告

在56°C加热30分钟制备。
该产品是在56°C加热30分钟而制备的。将血清加热至56°C 30分钟可灭活补体系统以进行免疫测定。热激活还会使培养物中其他未确定的细胞生长抑制剂失活。

分析報告

  • 内毒素和血红蛋白测试
  • 检测了是否存在细菌、病毒和支原体
  • 在无菌条件下用0.1微米膜过滤三次

儲存類別代碼

10 - Combustible liquids

水污染物質分類（WGK）

WGK 3

閃點（°F）

Not applicable

閃點（°C）

Not applicable

個人防護裝備

Eyeshields, Gloves

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

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Combining probiotic Phaeobacter inhibens DSM17395 and broad-host-range vibriophage KVP40 against fish pathogenic vibrios
Rasmussen BB, et al
Aquaculture (Amsterdam, Netherlands), 513, 734415-734415 (2019)
Sanjay Ghosh et al.
Nucleic acids research, 44(9), e84-e84 (2016-02-07)
Long non-coding RNAs (lncRNAs) have emerged as regulators of gene expression across metazoa. Interestingly, some lncRNAs function independently of their transcripts - the transcription of the lncRNA locus itself affects target genes. However, current methods of loss-of-function analysis are insufficient
Ming Guan et al.
American journal of translational research, 11(3), 1343-1358 (2019-04-12)
Aging and exposure to noise or ototoxic drugs are major causes of hair cell death leading to human hearing loss, and many agents have been developed to protect hair cells from apoptosis. Fetal bovine serum (FBS) is a fundamental ingredient
Deven Patel et al.
The Journal of biological chemistry, 291(17), 9322-9329 (2016-02-28)
During G1-phase of the cell cycle, normal cells respond first to growth factors that indicate that it is appropriate to divide and then later in G1 to the presence of nutrients that indicate sufficient raw material to generate two daughter
C Kerr et al.
Oncogene, 36(21), 2981-2990 (2016-12-13)
Type 2 transglutaminase (TG2) is an important cancer stem cell survival protein that exists in open and closed conformations. The major intracellular form is the closed conformation that functions as a GTP-binding GTPase and is required for cancer stem cell
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